Potnis Chinmay S, Grapperhaus Craig A, Gupta Gautam
Department of Chemistry, University of Louisville, Louisville, Kentucky 40292, United States.
Department of Chemical Engineering, University of Louisville, Louisville, Kentucky 40292, United States.
ACS Omega. 2023 Aug 21;8(35):32053-32059. doi: 10.1021/acsomega.3c04012. eCollection 2023 Sep 5.
There have been significant advances in the development of vaccines for the prevention of various infectious diseases in the last few decades. These vaccines are mainly composed of proteins and nucleic acids. Poor handling and storage, exposure to high temperatures that lead to enzymatic degradation, pH variation, and various other stresses can denature the proteins or nucleic acids present in any vaccine formulation. Therefore, it is necessary to maintain a proper environment to preserve the integrity of biospecimens. To overcome these challenges, we report a practical and user-friendly approach for sol-gels called "BioCaRGOS" that can stabilize heme proteins not only in the presence of degrading enzymes and acidic pH but simultaneously maintain stability at room temperature. Heme proteins, such as myoglobin and cytochrome c, have been used for this study.
在过去几十年里，用于预防各种传染病的疫苗研发取得了重大进展。这些疫苗主要由蛋白质和核酸组成。处理和储存不当、暴露于导致酶降解的高温、pH值变化以及各种其他压力因素，都可能使任何疫苗制剂中的蛋白质或核酸变性。因此，有必要维持适当的环境以保持生物样本的完整性。为了克服这些挑战，我们报告了一种实用且用户友好的溶胶-凝胶方法，称为“BioCaRGOS”，它不仅可以在存在降解酶和酸性pH值的情况下稳定血红素蛋白，还能在室温下保持稳定性。本研究使用了血红素蛋白，如肌红蛋白和细胞色素c。
