非小细胞肺癌中WNT信号通路两个效应元件NFATc2和PPARG基因表达水平的评估
Evaluation of Expression Levels of NFATc2 and PPARG Genes Two Effector Elements of WNT Pathway in Non-Small Cell Lung Carcinoma.
作者信息
Motamediyan Kaveh, Zafari Venus, Bornehdeli Soghra, Caner Ayşe, Asadi Milad, Hashemzadeh Shahryar, Firozi Mohammad Reza, Raeisi Mortaza
机构信息
Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Department of Basic Oncology of Health, Institute of Ege University, Izmir, Turkey.
出版信息
Adv Biomed Res. 2023 Jul 20;12:184. doi: 10.4103/abr.abr_185_22. eCollection 2023.
BACKGROUND
There is an emergency need in discovering an efficient profile of molecular biomarkers for early diagnosis of Non-small cell lung cancer (NSCLC). Transcription factors as important groups of regulators that are able to adjust the cell cycles have attracted the attention of most researchers recently. NFATc2 and PPARG are two important factors that have been selected for this project to assess their potential for being a biomarker for NSCLC.
MATERIALS AND METHODS
Here in this study, 50 NSCLC patients were included. During bronchoscopy, which was their routine diagnostic approach, we collected tumoral and marginal normal tissues. After the extraction of the total RNA from the tissues, cDNA was synthesized, and the transcriptional level of NFATc2 and PPARG was examined by quantitative real-time PCR. Subsequently, the data were analyzed by proper statistical analyses.
RESULTS
The mRNA expression of NFATc2 and PPARG were down-regulated in biopsy tissues of NSCLC patients compared with their pair marginal tissues ( were 0.0011 and <0.0001 respectively). Moreover, both of them had significant AUC (area under the curve) in the ROC curve analysis (0.65 for NFATc2 and 0.81 for PPARG, <0.05).
CONCLUSION
It appears that mRNA expression of NFATc2 and PPARG possesses the potential to be regarded as a diagnostic or prognostic biomarker for NSCLC.
背景
迫切需要发现用于非小细胞肺癌（NSCLC）早期诊断的有效分子生物标志物谱。转录因子作为能够调节细胞周期的重要调节因子组，最近引起了大多数研究人员的关注。NFATc2和PPARG是本项目中选择的两个重要因子，以评估它们作为NSCLC生物标志物的潜力。
材料与方法
本研究纳入了50例NSCLC患者。在作为其常规诊断方法的支气管镜检查期间，我们收集了肿瘤组织和边缘正常组织。从组织中提取总RNA后，合成cDNA，并通过定量实时PCR检测NFATc2和PPARG的转录水平。随后，通过适当的统计分析对数据进行分析。
结果
与配对的边缘组织相比，NSCLC患者活检组织中NFATc2和PPARG的mRNA表达下调（分别为0.0011和<0.0001）。此外，在ROC曲线分析中，它们两者都具有显著的曲线下面积（AUC）（NFATc2为0.65，PPARG为0.81，P<0.05）。
结论
看来NFATc2和PPARG的mRNA表达有潜力被视为NSCLC的诊断或预后生物标志物。
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