Craven N
Comp Immunol Microbiol Infect Dis. 1986;9(1):29-36. doi: 10.1016/0147-9571(86)90072-x.
The chemotactic effect of a variety of agents for bovine blood polymorphonuclear neutrophils (PMN) was evaluated in vitro in assays of migration under agarose. Activated serum and leukotriene B4 showed significant chemotactic activity whereas various bacterial products, formyl peptides, casein and platelet activating factor failed to attract bovine PMN. In vitro cultures of bovine mammary gland macrophages released chemotactic activity for homologous PMN when stimulated by addition of opsonised, killed Staphylococcus aureus, Escherichia coli or Zymosan or sterile E. coli culture filtrates or endotoxin. No significant activity was produced by unstimulated macrophages. Pharmacological levels of various inhibitors or arachidonic acid oxygenation had no significant effect on the generation of PMN chemoattractants by mammary macrophages.
在琼脂糖下迁移试验中，体外评估了多种试剂对牛血多形核中性粒细胞（PMN）的趋化作用。活化血清和白三烯B4显示出显著的趋化活性，而各种细菌产物、甲酰肽、酪蛋白和血小板活化因子未能吸引牛PMN。当添加调理过的、杀死的金黄色葡萄球菌、大肠杆菌或酵母聚糖或无菌大肠杆菌培养滤液或内毒素刺激时，牛乳腺巨噬细胞的体外培养物释放出对同源PMN的趋化活性。未刺激的巨噬细胞未产生显著活性。各种抑制剂或花生四烯酸氧化的药理水平对乳腺巨噬细胞产生PMN趋化因子没有显著影响。
