Politis I, Zhao X, McBride B W, Burton J H
Department of Animal and Poultry Science, University of Guelph, Ontario.
Can J Vet Res. 1991 Oct;55(4):321-4.
An in vitro model system in which polymorphonuclear leukocytes (PMN) migration under agarose was employed to examine the ability of mammary macrophages to release chemoattractants for PMN. Mammary macrophages were incubated in Hanks' balanced salt solution for up to 12 h in the presence of Staphylococcus aureus. The possibility that the chemotactic activity is mediated through release of interleukin 1 (IL-1) and prostaglandins (PGs) by mammary macrophages was investigated. The data showed that release of chemotactic activity peaked 6 h following addition of S. aureus in the culture medium of mammary macrophages. Very low levels of IL-1 were detected in the same culture medium. Addition of indomethacin, a PGs synthesis inhibitor, was ineffective in altering the chemotactic activity detected in the culture medium of macrophages. These data suggest that it is highly unlikely that the chemotactic activity is mediated through the production of IL-1 and PGs by the mammary macrophages.
利用一种体外模型系统，其中多形核白细胞（PMN）在琼脂糖下的迁移用于检测乳腺巨噬细胞释放PMN趋化因子的能力。将乳腺巨噬细胞在含有金黄色葡萄球菌的汉克斯平衡盐溶液中孵育长达12小时。研究了趋化活性通过乳腺巨噬细胞释放白细胞介素1（IL-1）和前列腺素（PGs）介导的可能性。数据显示，在乳腺巨噬细胞培养基中加入金黄色葡萄球菌后6小时，趋化活性释放达到峰值。在同一培养基中检测到极低水平的IL-1。加入PGs合成抑制剂吲哚美辛对改变巨噬细胞培养基中检测到的趋化活性无效。这些数据表明，趋化活性极不可能通过乳腺巨噬细胞产生IL-1和PGs来介导。
