College of Chemistry and Materials Science, Inner Mongolia Key Laboratory of Carbon Nanomaterials, Nano Innovation Institute (NII), Inner Mongolia Minzu University, Tongliao 028000, PR China.
ACS Biomater Sci Eng. 2023 Oct 9;9(10):5632-5638. doi: 10.1021/acsbiomaterials.3c00986. Epub 2023 Sep 11.
In this study, magnetic polyoxometalate-based metal-organic frameworks (FeO-POMOFs) were designed and applied to the enrichment of phosphopeptides. Thanks to the abundant metal oxide and metal ion sites, the material had a strong affinity for phosphopeptides. Simultaneously, the high amount of amino and guanidyl groups provided hydrophilicity and positive charge for phosphopeptide capture. By coupling with MS detection, the established platform possessed good reusability, high sensitivity (0.01 fmol), and high selectivity (α-casein/β-casein/bovine serum albumin = 1:1:5000). Furthermore, the method was successfully used for the detection of phosphopeptides in nonfat milk, human serum, saliva, and A549 cell lysate, showing great potential for practical application.
在这项研究中，设计了基于磁性多金属氧酸盐的金属有机骨架（FeO-POMOFs），并将其应用于磷酸肽的富集。由于丰富的金属氧化物和金属离子位点，该材料对磷酸肽具有很强的亲和力。同时，大量的氨基和胍基基团为磷酸肽的捕获提供了亲水性和正电荷。通过与 MS 检测相结合，所建立的平台具有良好的可重复使用性、高灵敏度（0.01 fmol）和高选择性（α-酪蛋白/β-酪蛋白/牛血清白蛋白 = 1:1:5000）。此外，该方法还成功地用于检测脱脂乳、人血清、唾液和 A549 细胞裂解物中的磷酸肽，显示出实际应用的巨大潜力。
