Rodriguez-Rios M, Rinaldi G, Megia-Fernandez A, Lilienkampf A, Robb C T, Rossi A G, Bradley M
EaStCHEM School of Chemistry, University of Edinburgh, David Brewster Road, EH9 3FJ Edinburgh, UK.
University of Edinburgh Centre for Inflammation Research, Queen's Medical Research Institute, 47 Little France Crescent, Edinburgh BioQuarter, Edinburgh EH16 4TJ, UK.
Chem Commun (Camb). 2023 Sep 28;59(78):11660-11663. doi: 10.1039/d3cc03634k.
Neutrophils are the first immune cells recruited for defence against invading pathogens; however, their dysregulated activation and subsequent release of the enzyme human neutrophil elastase is associated with several, inflammation-based, diseases. Herein, we describe a FRET-based, tri-branched (one quencher, three fluorophores) near infrared probe that provides an intense OFF/ON amplified fluorescence signal for specific detection of human neutrophil elastase. The probe allowed selective detection of activated neutrophils and labelling of neutrophil extracellular traps.
中性粒细胞是首批被招募来抵御入侵病原体的免疫细胞；然而，它们的失调激活以及随后人类中性粒细胞弹性蛋白酶的释放与几种基于炎症的疾病相关。在此，我们描述了一种基于荧光共振能量转移（FRET）的三分支（一个猝灭剂，三个荧光团）近红外探针，该探针为人类中性粒细胞弹性蛋白酶的特异性检测提供强烈的关/开放大荧光信号。该探针能够选择性地检测活化的中性粒细胞并标记中性粒细胞胞外诱捕网。
