Rios Maria R, Garoffolo Gloria, Rinaldi Giulia, Megia-Fernandez Alicia, Ferrari Silvia, Robb Calum T, Rossi Adriano G, Pesce Maurizio, Bradley Mark
EaStCHEM School of Chemistry, University of Edinburgh, David Brewster Road, EH9 3FJ Edinburgh, UK.
Chem Commun (Camb). 2021 Jan 5;57(1):97-100. doi: 10.1039/d0cc07028a.
A highly sensitive optical probe for the detection of activated neutrophils and Neutrophil Extracellular Traps (NETs) is reported. It is based on a triple-quenched, super-silent tri-branched probe that generates >20 fold increase in fluorescence upon cleavage. The probe was highly specific for human neutrophil elastase, a protease that mediates a variety of inflammatory diseases, and detected NETosis and neutrophil activation in in vitro differentiated neutrophils and isolated human neutrophils.
报道了一种用于检测活化中性粒细胞和中性粒细胞胞外诱捕网（NETs）的高灵敏度光学探针。它基于一种三重猝灭的超静音三分支探针，该探针在裂解后荧光增强超过20倍。该探针对人中性粒细胞弹性蛋白酶具有高度特异性，人中性粒细胞弹性蛋白酶是一种介导多种炎症性疾病的蛋白酶，可检测体外分化的中性粒细胞和分离的人中性粒细胞中的NETosis和中性粒细胞活化。
