Díaz-Sánchez Lidia, Zentella-Dehesa Alejandro, Castro-Torres Víctor Alberto, Silva-Jiménez Noemi, Jacobo-Herrera Nadia Judith, Martínez-Vázquez Mariano
Instituto de Química, Universidad Nacional Autónoma de México, C. Exterior, C. Universitaria, Coyoacán, 04510, CDMX, México (MMV).
Departamento de Bioquímica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán INCMNSZ., C. Vasco de Quiroga 15, Tlalpan, 14080 CDMX, México (NJJH).
Chem Biodivers. 2023 Oct;20(10):e202300893. doi: 10.1002/cbdv.202300893. Epub 2023 Sep 21.
The cytotoxic activity of combinations of masticadienonic (AMD) or 3αOH-hydroxy-masticadienonic (3αOH-AMD) acids with cisplatin (CDDP) was evaluated against PC3 prostate and HCT116 colon cancer cell lines. Combinations A (half the IC value), B (IC value), and C (twice the IC value) were tested at a 1 : 1 ratio. All AMD plus CDDP combinations demonstrated increased cytotoxic effect, as determined by the sulforhodamine B test, in both cell types. The best combination was B, which showed 93 % and 91 % inhibition of the proliferation of PC3 and HCT116 cells, respectively. It also increased apoptosis in the PC3 cell lines, as evaluated by flow cytometry. However, in vivo tests showed no additional activity from the AMD plus CDDP combinations. These results showed that the increased cytotoxic activity of the combinations in vitro did not reflect in vivo tests. All combinations of 3αOH-AMD plus CDDP exerted antagonistic effects in both cell types.
评估了乳香二烯酮酸（AMD）或3α-羟基乳香二烯酮酸（3αOH-AMD）与顺铂（CDDP）联合使用对PC3前列腺癌细胞系和HCT116结肠癌细胞系的细胞毒性活性。以1∶1的比例测试了组合A（IC值的一半）、组合B（IC值）和组合C（IC值的两倍）。通过磺酰罗丹明B试验测定，所有AMD加CDDP的组合在两种细胞类型中均表现出增强的细胞毒性作用。最佳组合是组合B，它分别对PC3和HCT116细胞的增殖显示出93%和91%的抑制率。通过流式细胞术评估，它还增加了PC3细胞系中的细胞凋亡。然而，体内试验表明AMD加CDDP的组合没有额外的活性。这些结果表明，组合在体外增加的细胞毒性活性在体内试验中并未体现。3αOH-AMD加CDDP的所有组合在两种细胞类型中均产生拮抗作用。
