Herrera-Jurado Erick Alejandro, Terán-Sánchez Estefanía De Jesús, Serrano-Contreras José Iván, Zepeda-Vallejo L Gerardo
Departamento de Química Orgánica, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prolongación de Carpio y Plan de Ayala s/n, Col. Santo Tomas., Delegación Miguel Hidalgo, Mexico City C.P. 11340, Mexico.
Department of Metabolism, Digestion and Reproduction, Section of Biomolecular Medicine, Faculty of Medicine, Imperial College London, South Kensington Campus, London SW7 2AZ, UK.
Molecules. 2025 May 16;30(10):2185. doi: 10.3390/molecules30102185.
(cuachalalate) is a medicinal plant widely used in traditional Mexican medicine for its anti-inflammatory, gastroprotective, and antimicrobial properties. In this study, we applied qualitative and quantitative NMR-based metabolomics profiling, combined with multivariate statistical analyses, including Principal Component Analysis (PCA), Partial Least Squares Discriminant Analysis (PLS-DA), and S-plots, to evaluate the chemical composition and authenticity of samples collected from different commercial sources sold in Mexico City. Metabolomic profiles in organic and aqueous extracts revealed highly similar spectral patterns among all collected samples, supporting the consistency of commercially available in Mexico. The presence of 3α-hydroxy-masticadienoic acid (3α-HMDA) and anacardic acids, biomarkers of the genus, was confirmed by H NMR in hexane extracts; in the aqueous extract they were not observed with the same analytical platform. These findings suggest that the traditional infusion method may not effectively extract the above-mentioned key bioactive compounds. This approach enhances quality control and ensures the reliability of products in the commercial market.
（古阿茶拉特）是一种药用植物，因其抗炎、胃保护和抗菌特性而在墨西哥传统医学中广泛使用。在本研究中，我们应用基于核磁共振的定性和定量代谢组学分析，并结合多变量统计分析，包括主成分分析（PCA）、偏最小二乘判别分析（PLS-DA）和S图，来评估从墨西哥城销售的不同商业来源收集的样品的化学成分和真伪。有机提取物和水提取物中的代谢组学图谱显示，所有收集的样品之间的光谱模式高度相似，这支持了墨西哥市售产品的一致性。己烷提取物中的1H NMR证实了3α-羟基乳香二烯酸（3α-HMDA）和漆树酸的存在，它们是该属的生物标志物；在水提取物中，使用相同的分析平台未观察到它们。这些发现表明，传统的冲泡方法可能无法有效地提取上述关键生物活性化合物。这种方法加强了质量控制，并确保了商业市场上产品的可靠性。
