Lin Kunning, Wang Lianghui, Zhang Yuanbiao
Key Laboratory of Global Change and Marine Atmospheric Chemistry, Third Institute of Oceanography, Ministry of Natural Resources, 361005 Xiamen, Fujian, China.
Key Laboratory of Global Change and Marine Atmospheric Chemistry, Third Institute of Oceanography, Ministry of Natural Resources, 361005 Xiamen, Fujian, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2024 Jan 5;304:123366. doi: 10.1016/j.saa.2023.123366. Epub 2023 Sep 6.
The presence of nitrate (NO) in the aquatic environment has raised a major concern for scientists and environmental managers. In this study, a smartphone-based resorcinol method was developed for the determination of NO in seawater. Simple custom-made devices were used in the method, and the reaction temperature, reaction time, and smartphone camera settings were optimized. Salinity variation did not show any major impact on the determination of NO using the proposed method, and hence the incorporation of a correction factor was also not required. The detection limit for this method was observed to be 1.3 µM, and the working range was observed to be 5-60 µM, with a relative standard deviation of 0.7% (5 µM, n = 7), which was adequate for the determination of NO in most estuarine and coastal seawater samples. The proposed method was compared with the frequently used vanadium chloride (VCl) reduction method under the same experimental conditions, and both methods were found to be beneficial. The proposed method procedure was simple and easy to use. It was successfully applied for the determination of NO in seawater samples, and the results showed that it was practical and can be used potentially for on-site analysis.
水生环境中硝酸盐（NO）的存在引起了科学家和环境管理者的重大关注。在本研究中，开发了一种基于智能手机的间苯二酚法用于测定海水中的NO。该方法使用了简单的定制设备，并对反应温度、反应时间和智能手机相机设置进行了优化。盐度变化对使用所提出方法测定NO没有显示出任何重大影响，因此也不需要纳入校正因子。观察到该方法的检测限为1.3 μM，工作范围为5 - 60 μM，相对标准偏差为0.7%（5 μM，n = 7），这对于大多数河口和沿海海水样品中NO的测定是足够的。在相同实验条件下，将所提出的方法与常用的氯化钒（VCl）还原法进行了比较，发现两种方法都有好处。所提出的方法步骤简单且易于使用。它成功地应用于海水样品中NO的测定，结果表明它是实用的，并且有可能用于现场分析。
