Yao Baohui, Tan Yuchen, An Kang, Kang Yukun, Hou Qiqi, Zhang Degang, Su Junhu
College of Grassland Science, Key Laboratory of Grassland Ecosystem (Ministry of Education), Gansu Agricultural University, Lanzhou 730070, China; Gansu Agricultural University-Massey University Research Centre for Grassland Biodiversity, Gansu Agricultural University, Lanzhou 730070, China.
College of Grassland Science, Key Laboratory of Grassland Ecosystem (Ministry of Education), Gansu Agricultural University, Lanzhou 730070, China; Gansu Agricultural University-Massey University Research Centre for Grassland Biodiversity, Gansu Agricultural University, Lanzhou 730070, China.
Comp Biochem Physiol Part D Genomics Proteomics. 2023 Dec;48:101143. doi: 10.1016/j.cbd.2023.101143. Epub 2023 Sep 6.
The gonads of seasonal breeding animals undergo periodic annual changes in morphology, physiological hormones, and gene expression levels. To clarify the regulatory mechanism of miRNAs in the seasonal testicular development and spermatogenesis of plateau zokors, the miRNA expression profiles in their testicles during breeding and non-breeding seasons were analyzed. In total, 447 miRNAs, including 366, 81, and 167 known, novel, and differentially expressed (DE) miRNAs, respectively, were determined in the testes. Compared to the non-breeding season, 90 DE miRNAs were upregulated and 77 DE miRNAs were downregulated during the breeding season. By analysing the miRNA and mRNA expression profiles, we predicted 2096 significant target mRNAs. According to the miRNA-mRNA interaction network, target mRNAs with DE miRNAs were related to testicular development and spermatogenesis. GO indicated that target mRNAs were enriched in spermatogenesis, cell differentiation, multicellular biological development, and flagellated sperm movement and were associated with regulating testicular development and spermatogenesis. KEGG suggested that target mRNAs were enriched in lipid and fructose metabolism and provided energy and material for spermatogenesis. The target mRNA of rno-miR-24-3p was determined to be Polyubiquitin-B (UBB). Our results provide a reference for revealing the mechanism by which miRNAs regulate testicular development and spermatogenesis in plateau zokors, which has important implications for understanding the regulation of seasonal reproduction in animals.
季节性繁殖动物的性腺在形态、生理激素和基因表达水平上经历周期性的年度变化。为了阐明微小RNA（miRNA）在高原鼢鼠季节性睾丸发育和精子发生中的调控机制，分析了其在繁殖季节和非繁殖季节睾丸中的miRNA表达谱。在睾丸中总共鉴定出447个miRNA，其中分别包括366个已知miRNA、81个新miRNA和167个差异表达（DE）miRNA。与非繁殖季节相比，繁殖季节有90个DE miRNA上调，77个DE miRNA下调。通过分析miRNA和mRNA表达谱，我们预测了2096个显著的靶mRNA。根据miRNA-mRNA相互作用网络，具有DE miRNA的靶mRNA与睾丸发育和精子发生有关。基因本体论（GO）表明，靶mRNA在精子发生、细胞分化、多细胞生物发育和鞭毛精子运动中富集，并与调节睾丸发育和精子发生有关。京都基因与基因组百科全书（KEGG）表明，靶mRNA在脂质和果糖代谢中富集，为精子发生提供能量和物质。确定rno-miR-24-3p的靶mRNA为多聚泛素-B（UBB）。我们的研究结果为揭示miRNA调控高原鼢鼠睾丸发育和精子发生的机制提供了参考，这对理解动物季节性繁殖的调控具有重要意义。
