School of Chemical Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan 38541, Republic of Korea.
Viyen Biotech LLP, Coimbatore-641034, Tamil Nadu, India; Department of Biotechnology, Sri Ramakrishna College of Arts & Science, Nava India, Coimbatore-641006, Tamil Nadu, India.
Environ Res. 2023 Dec 1;238(Pt 1):117109. doi: 10.1016/j.envres.2023.117109. Epub 2023 Sep 9.
The synthesis of polymer-encapsulated metal nanoparticles is a growing field of area due to their long-term uses in the development of new technologies. The present study describes the synthesis of chitosan/silver nanocomposite using kaempferol for anticancer and bactericidal activity. The formation of Kf-CS/Ag nanocomposite was confirmed by the development of a brown color and UV-absorbance around 438 nm. The IR study was utilized to determine the existence of Kf and CS in the synthesized nanocomposite. TEM analysis demonstrated that the synthesized nanocomposite have a predominantly uniform spherical shape and size ranges 7-10 nm. EDX spectrum showed the existence of Ag, C, and N elements in the nanocomposite material. Further, Kf-CS/Ag nanocomposite exhibited potential in vitro inhibitory property against triple-negative breast cancer (TNBC) cells and their IC values was found to be 53 μg/mL. Moreover, fluorescent assays such as DAPI and AO/EtBr confirmed the apoptosis induction ability of Kf-CS/Ag nanocomposite in MDA-MB-231 cells. The synthesized Kf-CS/Ag nanocomposite showed significant and dose-depended antibacterial property against S. aureus and P. aeruginosa. Thus, the obtained findings demonstrated that the synthesized nanocomposite can be potentially used to improve human health as biocidal nanocomposite in biomedical sectors.
聚合物封装金属纳米粒子的合成是一个快速发展的领域，因为它们在新技术的开发中有长期的应用。本研究描述了使用山奈酚合成壳聚糖/银纳米复合材料，以实现抗癌和杀菌活性。Kf-CS/Ag 纳米复合材料的形成通过形成棕色颜色和 438nm 左右的紫外吸收得到证实。IR 研究用于确定合成纳米复合材料中 Kf 和 CS 的存在。TEM 分析表明，合成的纳米复合材料具有主要均匀的球形形状和 7-10nm 的尺寸范围。EDX 光谱显示纳米复合材料中存在 Ag、C 和 N 元素。此外，Kf-CS/Ag 纳米复合材料在体外对三阴性乳腺癌（TNBC）细胞表现出潜在的抑制作用，其 IC 值为 53μg/mL。此外，DAPI 和 AO/EtBr 等荧光测定法证实了 Kf-CS/Ag 纳米复合材料在 MDA-MB-231 细胞中诱导凋亡的能力。合成的 Kf-CS/Ag 纳米复合材料对金黄色葡萄球菌和铜绿假单胞菌表现出显著的、剂量依赖性的抗菌性能。因此，研究结果表明，所合成的纳米复合材料可作为生物杀灭纳米复合材料，在生物医学领域中潜在地用于改善人类健康。
