Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore 029, Tamilnadu, India.
Department of Biotechnology, Kongunadu Arts and Science College, Coimbatore 029, Tamilnadu, India.
Int J Biol Macromol. 2020 Dec 1;164:2779-2787. doi: 10.1016/j.ijbiomac.2020.08.047. Epub 2020 Aug 7.
The present study reports an eco-friendly synthesis of chitosan/zinc oxide (CS/ZnO) nanocomposite using S. lycopersicum leaf extract by a bio-inspired method. The synthesized CS/ZnO nanocomposite was characterized by using UV-visible spectroscopy, X-ray diffraction (XRD), field emission-scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), energy dispersive X-ray spectroscopy (EDS), fourier transform infrared spectroscopy (FTIR) techniques. The XRD analysis revealed wurtzite crystalline structure of CS/ZnO nanocomposite. Electron microscopy images showed agglomeration of CS/ZnO nanocomposite having spherical shaped structure with an average size of 21-47 nm. The observed bands around 400-500 cm in the IR spectrum indicated the presence of metal‑oxygen bond, whereas bands at 1512 and 1745 cm indicated the presence of amine groups (-NH) which confirms the presence of CS in the CS/ZnO nanocomposite. The synthesized nanocomposite showed potential antibacterial activity against skin infection causing S. aureus and the mechanism of bactericidal activity was confirmed by using FE-SEM. The CS/ZnO nanocomposite incorporated cotton fabrics also exhibited antibacterial activity against S. aureus, B. subtilis and E. coli. Furthermore, CS/ZnO nanocomposite acted as photocatalyst for the degradation of Congo red under sunlight irradiation. In conclusion, as-synthesized CS/ZnO nanocomposite can be used as bactericidal agent in textile industries and also as photocatalyst for dye degradation.
本研究采用生物启发法，利用番茄叶提取物，报告了壳聚糖/氧化锌（CS/ZnO）纳米复合材料的环保合成方法。采用紫外-可见分光光度法、X 射线衍射（XRD）、场发射扫描电子显微镜（FE-SEM）、透射电子显微镜（TEM）、能量色散 X 射线光谱（EDS）、傅里叶变换红外光谱（FTIR）技术对合成的 CS/ZnO 纳米复合材料进行了表征。XRD 分析表明 CS/ZnO 纳米复合材料具有纤锌矿晶体结构。电子显微镜图像显示 CS/ZnO 纳米复合材料存在团聚现象，具有球形结构，平均尺寸为 21-47nm。IR 光谱中 400-500cm 左右的观察到的谱带表明存在金属-氧键，而 1512 和 1745cm 左右的谱带表明存在胺基（-NH），这证实了 CS 在 CS/ZnO 纳米复合材料中的存在。合成的纳米复合材料对引起皮肤感染的金黄色葡萄球菌具有潜在的抗菌活性，并且通过 FE-SEM 证实了杀菌活性的机制。掺入 CS/ZnO 纳米复合材料的棉织物也对金黄色葡萄球菌、枯草芽孢杆菌和大肠杆菌表现出抗菌活性。此外，CS/ZnO 纳米复合材料在阳光照射下可作为刚果红降解的光催化剂。总之，合成的 CS/ZnO 纳米复合材料可用于纺织工业中的杀菌剂，也可用作光催化剂用于染料降解。
