Seo Hyeyeong, Ahn Yu-Jin, Seo Huiwon, Seo Ahreum, Lee Hayeon, Lee Seok-Hee, Shon Won-Jun, Park Yooheon
Department of Food Science and Biotechnology, Dongguk University, Goyang, 10326, Republic of Korea.
Department of Conservative Dentistry, Dental Research Institute and School of Dentistry, Seoul National University, Seoul, 03080, Republic of Korea.
Chemosphere. 2023 Dec;343:140104. doi: 10.1016/j.chemosphere.2023.140104. Epub 2023 Sep 9.
Resin-based dental composites have been developed to restore decayed teeth or modify tooth color due to their excellent physical and chemical properties. Such composites may have intrinsic toxicity due to components released into the mouth during the early stage of polymerization, and afterward as a result of erosion or material decomposition. In addition, resin-based dental composites have potential environmental pollutant by elution of monomers and degradation. Since certain monomers of resin matrices are synthesized from bisphenol A (BPA), which acts as an estrogenic endocrine disruptor, these resin matrices may have estrogenic activity. Therefore, the estrogenic endocrine-disrupting activity of various dental composites should be evaluated. In this study, we evaluated the estrogenic endocrine-disrupting activity of 10 resin composites by using a BRET-based estrogen receptor (ER)α and ERβ dimerization assays and ER transactivation assay. BPA, BisDMA, BisGMA, BisEMA, TEGDMA, HMBP, and DMPA mediated ERα dimerization, and BPA, BisDMA, and DMPA also mediated ERβ dimerization. Except for UDMA and CQ, all the compounds were identified as estrogen agonists or antagonists. In-depth information for the safe use of dental composites was acquired, and it was confirmed how the component of dental composites acts in the ER signaling pathway. Further studies on the low-dose and long-term release of these compounds are needed to ensure the safe use of these resin-based dental composites.
基于树脂的牙科复合材料因其优异的物理和化学性能而被开发用于修复龋齿或改变牙齿颜色。由于在聚合早期以及随后由于侵蚀或材料分解而释放到口腔中的成分，此类复合材料可能具有内在毒性。此外，基于树脂的牙科复合材料通过单体的洗脱和降解而具有潜在的环境污染物。由于树脂基体的某些单体是由双酚A（BPA）合成的，双酚A是一种雌激素内分泌干扰物，这些树脂基体可能具有雌激素活性。因此，应评估各种牙科复合材料的雌激素内分泌干扰活性。在本研究中，我们通过基于BRET的雌激素受体（ER）α和ERβ二聚化测定以及ER反式激活测定来评估10种树脂复合材料的雌激素内分泌干扰活性。BPA、BisDMA、BisGMA、BisEMA、TEGDMA、HMBP和DMPA介导ERα二聚化，BPA、BisDMA和DMPA也介导ERβ二聚化。除UDMA和CQ外，所有化合物均被鉴定为雌激素激动剂或拮抗剂。获得了关于牙科复合材料安全使用的深入信息，并证实了牙科复合材料的成分在ER信号通路中的作用方式。需要对这些化合物的低剂量和长期释放进行进一步研究，以确保这些基于树脂的牙科复合材料的安全使用。
