Department of Computer Engineering, Toyohashi University of Technology, Aichi 441-8580, Japan.
Department of Computer Engineering, Toyohashi University of Technology, Aichi 441-8580, Japan
eNeuro. 2023 Sep 25;10(9). doi: 10.1523/ENEURO.0141-23.2023. Print 2023 Sep.
In neurophysiology, achieving precise correlation between physiological responses and anatomic structures is a significant challenge. Therefore, the accuracy of the electrode marking method is crucial. In this study, we describe a tungsten-deposition method, in which tungsten oxide is generated by applying biphasic current pulses to conventional tungsten electrodes. The electrical current used was 40-50 μA, which is similar to that used in electrical microstimulation experiments. The size of the markings ranged from 10 to 100 μm, corresponding to the size of the electrode tip, which is smaller than that of existing marking methods. Despite the small size of the markings, detection is easy as the marking appears in bright red under dark-field observation after Nissl staining. This marking technique resulted in low tissue damage and was maintained for at least two years. The feasibility of this method was tested in mouse and macaque brains.
在神经生理学中，实现生理反应与解剖结构之间的精确相关是一项重大挑战。因此，电极标记方法的准确性至关重要。在本研究中，我们描述了一种钨沉积方法，其中通过向常规钨电极施加双相电流脉冲来生成氧化钨。所使用的电流为 40-50μA，与电微刺激实验中使用的电流相似。标记的大小为 10 到 100μm，与电极尖端的大小相对应，比现有标记方法的标记更小。尽管标记很小，但由于在 Nissl 染色后暗场观察时标记呈亮红色，因此很容易检测到。这种标记技术造成的组织损伤较小，并且至少可以维持两年。在小鼠和猕猴大脑中测试了这种方法的可行性。
