College of Agronomy, Qingdao Agricultural University, Qingdao, 266109, China.
The Characteristic Laboratory of Crop Germplasm Innovation and Application, Provincial Department of Education, College of Agronomy, Qingdao Agricultural University, Qingdao, 266109, China.
BMC Genomics. 2023 Sep 11;24(1):537. doi: 10.1186/s12864-023-09567-z.
The mechanism of grain development in elite maize breeding lines has not been fully elucidated. Grain length, grain width and grain weight are key components of maize grain yield. Previously, using the Chinese elite maize breeding line Chang7-2 and its large grain mutant tc19, we characterized the grain size developmental difference between Chang7-2 and tc19 and performed transcriptomic analysis.
In this paper, using Chang7-2 and tc19, we performed comparative transcriptomic, proteomic and metabolomic analyses at different grain development stages. Through proteomics analyses, we found 2884, 505 and 126 differentially expressed proteins (DEPs) at 14, 21 and 28 days after pollination, respectively. Through metabolomics analysis, we identified 51, 32 and 36 differentially accumulated metabolites (DAMs) at 14, 21 and 28 days after pollination, respectively. Through multiomics comparative analysis, we showed that the phenylpropanoid pathways are influenced at transcriptomic, proteomic and metabolomic levels in all the three grain developmental stages.
We identified several genes in phenylpropanoid biosynthesis, which may be related to the large grain phenotype of tc19. In summary, our results provided new insights into maize grain development.
优质玉米育种系中谷物发育的机制尚未完全阐明。粒长、粒宽和粒重是玉米粒产量的关键组成部分。此前，我们使用中国优质玉米育种系昌 7-2 及其大粒突变体 tc19，对昌 7-2 和 tc19 之间的粒大小发育差异进行了表征，并进行了转录组分析。
本文以昌 7-2 和 tc19 为材料，在不同的籽粒发育阶段进行了比较转录组、蛋白质组和代谢组分析。通过蛋白质组学分析，我们在授粉后 14、21 和 28 天分别发现了 2884、505 和 126 个差异表达蛋白（DEPs）。通过代谢组学分析，我们在授粉后 14、21 和 28 天分别鉴定出 51、32 和 36 个差异积累代谢物（DAMs）。通过多组学比较分析，我们表明在三个籽粒发育阶段，苯丙烷生物合成途径在转录组、蛋白质组和代谢组水平上受到影响。
我们鉴定了苯丙烷生物合成中的几个基因，这些基因可能与 tc19 的大粒表型有关。总之，我们的研究结果为玉米籽粒发育提供了新的见解。
