Virology Section, Department of Life Sciences, Shiv Nadar Institution of Eminence, Gautam Buddha Nagar, India.
Translational Health Science and Technology Institute, NCR Biotech Science Cluster, Faridabad, India.
J Viral Hepat. 2023 Dec;30(12):959-969. doi: 10.1111/jvh.13885. Epub 2023 Sep 11.
Hepatitis E is a disease associated with acute inflammation of the liver. It is related to several dysregulated metabolic pathways and alterations in the concentration of several metabolites. However, longitudinal analysis of the alterations in metabolites and lipids is generally lacking. This study investigated the changes in levels of metabolites and lipids over time in sera from men with acute hepatitis E compared to healthy controls similar in age and gender. Untargeted measurement of levels of various metabolites and lipids was done using mass spectrometry on 65 sera sequentially sampled from 14 patients with acute hepatitis E and 25 serum samples from five controls. Temporal changes in intensities of metabolites and lipids were determined over different times at 3-day periods for the hepatitis E virus (HEV) group. In carbohydrate metabolism, glucose levels, fructose 1-6-bisphosphate and ribulose-5-phosphate were increased in the HEV-infected persons compared to the healthy controls. HEV infection is significantly associated with decreased levels of inosine, guanosine, adenosine and urate in purine metabolism and thymine, uracil and β-aminoisobutyrate in pyrimidine metabolism. Glutamate, alanine and valine levels were significantly lower in the HEV group than in healthy individuals. Homogentisate of tyrosine metabolism and cystathionine of serine metabolism were increased, whereas kynurenate of tryptophan metabolism decreased in the HEV group. Metabolites of the bile acid biosynthesis, urea cycle (arginine and citrulline) and ammonia recycling (urocanate) were significantly altered. Co-enzymes, pantothenate and pyridoxal, and co-factors, lipoamide and FAD, were elevated in the HEV group. The acylcarnitines, sphingomyelins, phosphatidylcholine (PC), phosphatidylethanolamine (PE), lysoPC and lysoPE tended to be lower in the HEV group. In conclusion, acute hepatitis E is associated with altered metabolite and lipid profiles, significantly increased catabolism of carbohydrates, purines/pyrimidines and amino acids, and decreased levels of several glycerophospholipids.
戊型肝炎是一种与肝脏急性炎症相关的疾病。它与几种代谢途径失调和几种代谢物浓度改变有关。然而，关于代谢物和脂质的纵向分析通常是缺乏的。本研究比较了年龄和性别相匹配的急性戊型肝炎患者和健康对照者血清中代谢物和脂质随时间的变化。使用质谱法对 14 例急性戊型肝炎患者和 25 例健康对照者的 65 份血清样本进行了各种代谢物和脂质水平的非靶向测量。在戊型肝炎病毒（HEV）组中，以 3 天为一个周期，分别在不同时间测定代谢物和脂质的强度变化。与健康对照组相比，HEV 感染者的糖水平、果糖 1-6-二磷酸和核酮糖 5-磷酸增加。HEV 感染与嘌呤代谢中的肌苷、鸟苷、腺苷和尿酸以及嘧啶代谢中的胸腺嘧啶、尿嘧啶和β-氨基异丁酸水平降低显著相关。HEV 组谷氨酸、丙氨酸和缬氨酸水平明显低于健康个体。酪氨酸代谢的同型戊二酸和丝氨酸代谢的胱硫醚增加，而色氨酸代谢的犬尿氨酸减少。胆汁酸生物合成、尿素循环（精氨酸和瓜氨酸）和氨循环（尿刊酸）的代谢物明显改变。辅酶、泛酸和吡哆醛以及辅因子、硫辛酰胺和 FAD 在 HEV 组中升高。酰基肉碱、神经鞘磷脂、磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、溶血 PC 和溶血 PE 在 HEV 组中趋于降低。总之，急性戊型肝炎与代谢物和脂质谱改变有关，碳水化合物、嘌呤/嘧啶和氨基酸的分解代谢显著增加，几种甘油磷脂的水平降低。
