Department of Obstetrics, Tongde Hospital of Zhejiang Provincial, Hangzhou, 310012, China.
Department of Obstetrics and Gynecology, The Second Affiliated Hospital of Zhejiang Chinese Medical University, Xinhua Hospital of Zhejiang Province, Hangzhou, 310005, China.
Biochem Genet. 2024 Jun;62(3):1742-1761. doi: 10.1007/s10528-023-10506-6. Epub 2023 Sep 12.
Existing research has confirmed the dysregulation of circular RNA (circRNA) in a wide variety of human diseases. Thus, in this study, we explored the potential mechanism of circRNA_0088196 in preeclampsia (PE). We performed quantitative real-time PCR to examine circRNA_0088196 expression and verified the function of circRNA_0088196 in vitro using CCK-8, TUNEL, flow cytometry, and Western blotting analyses. Additionally, we studied the mechanism using dual-luciferase reporter gene experiments. The results of our research revealed the up-regulation of circRNA_0088196 in PE patients' placentas and Heat Shock 70 kDa Protein 5 (HSPA5)-stimulated trophoblast (HTR-8/SVneo) cells. An investigation of the mechanism also showed that there was a binding between miR-379-5p and circRNA_0088196. Additionally, circRNA_0088196 inhibited HTR-8/SVneo cell proliferation and promoted cell apoptosis via the miR-337-3p/HSPA5 axis, thereby facilitating PE. In vivo experiments indicated that circRNA_0088196 regulated HTR-8/SVneo cell production through miR-379-5p. Overall, the findings of this study illustrate that circRNA_0088196 interference promotes cell apoptosis and inhibits HTR-8/SVneo proliferation via the miR-379-5p/HSPA5 axis, thereby accelerating the development of PE.
已有研究证实，环状 RNA（circRNA）在多种人类疾病中失调。因此，本研究探讨了 circRNA_0088196 在子痫前期（PE）中的潜在作用机制。我们通过定量实时 PCR 检测 circRNA_0088196 的表达，并通过 CCK-8、TUNEL、流式细胞术和 Western blot 分析在体外验证 circRNA_0088196 的功能。此外，我们还通过双荧光素酶报告基因实验研究了其作用机制。研究结果显示，PE 患者胎盘和热休克 70 kDa 蛋白 5（HSPA5）刺激滋养细胞（HTR-8/SVneo）中 circRNA_0088196 表达上调。机制研究还表明，miR-379-5p 与 circRNA_0088196 之间存在结合。此外，circRNA_0088196 通过 miR-337-3p/HSPA5 轴抑制 HTR-8/SVneo 细胞增殖并促进细胞凋亡，从而促进 PE 的发生。体内实验表明，circRNA_0088196 通过 miR-379-5p 调节 HTR-8/SVneo 细胞的产生。综上所述，本研究结果表明，circRNA_0088196 干扰通过 miR-379-5p/HSPA5 轴促进细胞凋亡并抑制 HTR-8/SVneo 增殖，从而加速 PE 的发生发展。
