Jia Miaomiao, Li Ping, Zhang Junyan, Chen Zhongwen, Gao Lei, Sun Yangming, Zhang Xiaofei, Yan Yong, Zhu Guoying
Jiaxing Key Laboratory of Pathogenic Microbiology, Jiaxing Center for Disease Control and Prevention, Jiaxing, China.
Institute of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.
Foodborne Pathog Dis. 2023 Oct;20(10):467-476. doi: 10.1089/fpd.2023.0051. Epub 2023 Sep 12.
is a primary cause of foodborne diseases, and the increasing prevalence of carrying plasmids, which confer colistin resistance to , poses significant global health concerns. As the frequency of occurrence of the gene is increasing globally, we studied the prevalence of in clinical isolates by analyzing 195 clinical strains isolated in 2020. Of the 195 isolates, 41 isolates were resistant to colistin. We found in two strains ( Typhimurium ZJJX20006 and Kentucky ZJJX20014), which we analyzed in detail via whole-genome sequencing and antibiotic susceptibility testing. Two strains displayed resistance to ampicillin, ampicillin-sulbactam, tetracycline, chloramphenicol, and cotrimoxazole, while ZJJX20006 displayed resistance to colistin and ZJJX20014 was sensitive. Genomic analysis revealed that these strains had plasmid-encoded in IncHI2 plasmids, which were not similar to the -IncX4 identified in 2016. These two strains also harbored other drug resistance genes, including and . Our findings may help clarify the molecular mechanisms of dissemination among strains in Jiaxing City and offer insights into the evolution of in .
是食源性疾病的主要原因，携带赋予对黏菌素耐药性的质粒的情况日益普遍，这引起了全球重大的健康关注。随着全球范围内该基因出现频率的增加，我们通过分析2020年分离的195株临床菌株，研究了临床分离株中该菌的流行情况。在这195株分离株中，有41株对黏菌素耐药。我们在两株菌（鼠伤寒沙门氏菌ZJJX20006和肯塔基沙门氏菌ZJJX20014）中发现了该菌，我们通过全基因组测序和抗生素敏感性测试对其进行了详细分析。两株菌对氨苄西林、氨苄西林 - 舒巴坦、四环素、氯霉素和复方新诺明耐药，而ZJJX20006对黏菌素耐药，ZJJX20014敏感。基因组分析表明，这些菌株在IncHI2质粒中含有质粒编码的该菌，这与2016年鉴定的该菌 - IncX4不同。这两株菌还携带其他耐药基因，包括和。我们的研究结果可能有助于阐明该菌在嘉兴市菌株中传播的分子机制，并为该菌在中的进化提供见解。
