Marquette University, Biological Sciences Department, Milwaukee, WI, USA.
Can J Microbiol. 2024 Jan 1;70(1):15-31. doi: 10.1139/cjm-2023-0030. Epub 2023 Sep 12.
Cold stress is an important factor limiting rice production and distribution. Identifying factors that contribute to cold tolerance in rice is of primary importance. While some plant specific genetic factors involved in cold tolerance have been identified, the role of the rice microbiome remains unexplored. In this study, we evaluated the influence of plant growth promoting bacteria (PGPB) with the ability of phosphate solubilization on rice cold tolerance and survival. To reach this goal, inoculated and uninoculated 2-week-old seedlings were cold stressed and evaluated for survival and other phenotypes such as electrolyte leakage (EL) and necessary elements for cold tolerance. The results of this study showed that of the five bacteria, , improved both and varietal plants' survival and decreased EL, indicating increased membrane integrity. We observed different possible cold tolerance mechanisms in and plants such as increases in proline and reduced glutathione levels, respectively. This bacterium also improved the shoot growth of cold exposed plants during the recovery period. This study confirmed the host genotype dependent activity of and indicated that there is an interaction between specific plant genes and bacterial genes that causes different plant responses to cold stress.
冷胁迫是限制水稻生产和分布的重要因素。鉴定导致水稻耐寒性的因素至关重要。虽然已经确定了一些与耐寒性相关的植物特异性遗传因素，但水稻微生物组的作用仍未得到探索。在这项研究中，我们评估了具有解磷能力的植物生长促进细菌 (PGPB) 对水稻耐寒性和生存能力的影响。为了达到这个目标，我们对接种和未接种的 2 周龄幼苗进行了冷胁迫处理，并评估了它们的存活率和其他表型，如电解质渗漏 (EL) 和耐寒所需的元素。研究结果表明，在这 5 种细菌中， 提高了 和 品种植物的存活率，降低了 EL，表明细胞膜的完整性增加。我们观察到 和 植物可能存在不同的耐寒机制，如脯氨酸和还原型谷胱甘肽水平的增加。这种细菌还改善了冷暴露后 植物在恢复期的芽生长。这项研究证实了 宿主基因型依赖性的活性，并表明特定植物基因和细菌基因之间存在相互作用，导致植物对冷胁迫产生不同的反应。
