Marquette Universitygrid.259670.f, Biological Sciences Department, Milwaukee, Wisconsin, USA.
Microbiol Spectr. 2022 Aug 31;10(4):e0278721. doi: 10.1128/spectrum.02787-21. Epub 2022 Jul 11.
Asian rice is one of the most important crops because it is a staple food for almost half of the world's population. To have production of rice keep pace with a growing world population, it is anticipated that the use of fertilizers will also need to increase, which may cause environmental damage through runoff impacts. An alternative strategy to increase crop yield is the use of plant growth-promoting bacteria. Thousands of microbial species can exist in association with plant roots and shoots, and some are critical to the plant's survival. We isolated 140 bacteria from two distantly related rice accessions and investigated whether their impact on the growth of four different rice accessions. The bacterial isolates were screened for their ability to solubilize phosphate, a known plant growth-promoting characteristic, and 25 isolates were selected for further analysis. These 25 phosphate-solubilizing isolates were also able to produce other potentially growth-promoting factors. Five of the most promising bacterial isolates were chosen for whole-genome sequencing. Four of these bacteria, isolates related to Pseudomonas mosselii, a sp., Paenibacillus rigui, and Paenibacillus graminis, improved root and shoot growth in a rice genotype-dependent manner. This indicates that while bacteria have several known plant growth-promoting functions, their effects on growth parameters are rice genotype dependent and suggest a close relationship between plants and their microbial partners. In this study, endophytic bacterial isolates from roots and shoots of two distantly related rice accessions were characterized phenotypically and genotypically. From the isolated bacterial species, five of the most promising plant growth-promoting bacteria were selected to test their abilities to enhance growth of the four rice accessions. Interestingly, plant growth enhancement was both bacterial isolate specific and plant genotype specific. However, the positive interactions between plant and bacteria could not easily be predicted because rice growth-promoting bacteria isolated from their host plants did not necessarily stimulate growth of their own host.
亚洲稻是最重要的作物之一，因为它是全球近一半人口的主食。为了使水稻产量跟上不断增长的世界人口，预计肥料的使用也将增加，这可能会通过径流影响对环境造成破坏。提高作物产量的另一种策略是使用植物生长促进细菌。成千上万的微生物物种可以与植物的根和茎共生，其中一些对植物的生存至关重要。我们从两个远缘水稻品种中分离出 140 种细菌，并研究了它们对四种不同水稻品种生长的影响。这些细菌分离物被筛选出其溶解磷酸盐的能力，这是一种已知的促进植物生长的特性，有 25 个分离物被选中进行进一步分析。这 25 个溶磷分离物还能够产生其他潜在的生长促进因子。选择最有前途的五个细菌分离物进行全基因组测序。这四种细菌中的四种，与假单胞菌属 mosselii、Paenibacillus rigui 和 Paenibacillus graminis 相关的细菌分离物，以依赖于水稻基因型的方式促进根和茎的生长。这表明，虽然细菌具有几种已知的促进植物生长的功能，但它们对生长参数的影响依赖于水稻基因型，并表明植物与其微生物伙伴之间存在密切关系。在这项研究中，从两个远缘水稻品种的根和茎中分离出内生细菌，从表型和基因型两方面对其进行了表征。从分离出的细菌种类中，选择了五种最有前途的植物生长促进细菌来测试它们增强四个水稻品种生长的能力。有趣的是，植物生长的增强既具有细菌分离物特异性，也具有植物基因型特异性。然而，植物和细菌之间的积极相互作用并不容易预测，因为从宿主植物中分离出的促进水稻生长的细菌不一定能刺激其自身宿主的生长。
