Department of Environmental Health and Technology, School of Public Health, Walailak University, Nakhon Si Thammarat, Thailand; Excellent Center for Dengue and Community Public Health (EC for DACH), Walailak University, Nakhon Si Thammarat, Thailand; Center of Excellence in Microbial Technology for Marine Pollution Treatment (MiTMaPT), Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.
Center of Excellence in Microbial Technology for Marine Pollution Treatment (MiTMaPT), Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand; Center of Excellence on Hazardous Substance Management (HSM), Chulalongkorn University, Bangkok, Thailand.
J Hazard Mater. 2023 Oct 15;460:132507. doi: 10.1016/j.jhazmat.2023.132507. Epub 2023 Sep 7.
Polyethylene terephthalate (PET), a petroleum-based plastic, and polylactic acid (PLA), a biobased plastic, have a similar visual appearance thus they usually end up in municipal waste treatment facilities. The objective of this project was to develop an effective PET and PLA waste treatment process that involves pretreatment with deep eutectic solvent (DES) followed by biodegradation with a plastic-degrading bacterial consortium in a composting system. The DES used was a mixture of choline chloride and glycerol, while the bacterial strains (Chitinophaga jiangningensis EA02, Nocardioides zeae EA12, Stenotrophomonas pavanii EA33, Gordonia desulfuricans EA63, Achromobacter xylosoxidans A9 and Mycolicibacterium parafortuitum J101) used to prepare the bacterial consortium were selected based on their ability to biodegrade PET, PLA, and plasticizer. The plastic samples (a PET bottle, PLA cup, and PLA film) were pretreated with DES through a dip-coating method. The DES-coated plastic samples exhibited higher surface wettability and biofilm formation, indicating that DES increases the hydrophilicity of the plastic and facilitates bacterial attachment to the plastic surface. The combined action of DES pretreatment and bioaugmentation with a plastic-degrading bacterial consortium led to improved degradation of PET and PLA samples in various environments, including aqueous media at ambient temperature, lab-scale traditional composting, and pilot-scale composting.
聚对苯二甲酸乙二醇酯（PET）和聚乳酸（PLA）是两种常见的塑料，它们都是基于石油的塑料和生物基塑料，外观相似，因此通常最终会进入城市垃圾处理设施。本项目的目的是开发一种有效的 PET 和 PLA 废物处理工艺，该工艺涉及使用深共熔溶剂（DES）进行预处理，然后在堆肥系统中使用塑料降解细菌混合物进行生物降解。所使用的 DES 是氯化胆碱和甘油的混合物，而用于制备细菌混合物的细菌菌株（解几丁质沙雷氏菌 EA02、玉米诺卡氏菌 EA12、施氏假单胞菌 EA33、脱硫戈登氏菌 EA63、木糖氧化无色杆菌 A9 和偶发分枝杆菌 J101）是根据其降解 PET、PLA 和增塑剂的能力选择的。通过浸涂法用 DES 对塑料样品（一个 PET 瓶、一个 PLA 杯和一个 PLA 薄膜）进行预处理。DES 涂层的塑料样品表现出更高的表面润湿性和生物膜形成能力，表明 DES 提高了塑料的亲水性，并促进了细菌附着在塑料表面。DES 预处理和添加塑料降解细菌混合物的联合作用导致 PET 和 PLA 样品在各种环境中的降解得到改善，包括环境温度下的水介质、实验室规模的传统堆肥和中试规模的堆肥。
