State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
State Key Laboratory of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China; School of Biotechnology and Key Laboratory of Industrial Biotechnology Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, China.
Sci Total Environ. 2022 Feb 20;808:152107. doi: 10.1016/j.scitotenv.2021.152107. Epub 2021 Dec 2.
Polyethylene terephthalate (PET) is a general plastic that produces a significant amount of waste due to its non-biodagradable properties. We obtained four bacteria (Stenotrophomonas pavanii JWG-G1, Comamonas thiooxydans CG-1, Comamonas koreensis CG-2 and Fulvimonas soli GM-1) that utilize PET as a sole carbon source through a novel stepwise screening and verification strategy. PET films pretreated with S. pavanii JWG-G1 exhibited weight loss of 91.4% following subsequent degradation by Thermobifida fusca cutinase (TfC). S. pavanii JWG-G1 was able to colonize the PET surface and maintain high cell viability (over 50%) in biofilm, accelerating PET degradation. Compared with PET films with no pretreatment, pretreatment with S. pavanii JWG-G1 caused the PET surface to be significantly rougher with greater hydrophilicity (contact angle of 86.3 ± 2° vs. 96.6 ± 2°), providing better opportunities for TfC to contact and act on PET. Our study indicates that S. pavanii JWG-G1 could be used as a novel pretreatment for efficiently accelerating PET biodegradation by TfC.
聚对苯二甲酸乙二醇酯（PET）是一种通用塑料，由于其不可生物降解的特性，会产生大量的废物。我们通过一种新颖的逐步筛选和验证策略，获得了四种能够将 PET 作为唯一碳源利用的细菌（产碱普罗威登斯菌 JWG-G1、硫杆菌 CG-1、朝鲜假单胞菌 CG-2 和地噬盐单胞菌 GM-1）。经过随后的解木聚糖酶（TfC）降解，用产碱普罗威登斯菌 JWG-G1 预处理的 PET 薄膜的失重率达到 91.4%。产碱普罗威登斯菌 JWG-G1 能够在生物膜表面定植并保持高细胞活力（超过 50%），从而加速 PET 的降解。与未经预处理的 PET 薄膜相比，用产碱普罗威登斯菌 JWG-G1 预处理后，PET 表面明显变得更粗糙，亲水性更强（接触角为 86.3 ± 2° vs. 96.6 ± 2°），为 TfC 接触和作用于 PET 提供了更好的机会。我们的研究表明，产碱普罗威登斯菌 JWG-G1 可用作一种新型预处理方法，通过 TfC 有效地加速 PET 的生物降解。
