Yang Chao, Dong Xingxiao, Zhang Xueli, Bi Changhao
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China.
School of Biological Engineering, Dalian Polytechnic University, Dalian 116034, Liaoning, China.
Sheng Wu Gong Cheng Xue Bao. 2022 Nov 25;38(11):4132-4145. doi: 10.13345/j.cjb.220566.
Precise and efficient manipulation of gene expression or rewriting genome sequence is the research hotspots of genome editing, and it is also the core enabling technology contributing to the rapid development of industrial biotechnology. Genome editing technology has experienced three stages of development, from zinc finger nuclease (ZFNs), to transcription activator like effector nuclease (TALEN) and Cas nuclease. Currently, vigorous development of CRISPR/Cas has enabled researchers establish a series of first-generation and second-generation Cas-based genome editing technologies. This contributed to the establishment and optimization for prokaryotic chassis such as or eukaryotic chassis such as . This paper summarizes the current development and application of industrial biotechnology using conventional chassis cells, and prospects future development trend with the aim to facilitate researchers to optimize industrial biotechnology and its potential applications.
精确且高效地操纵基因表达或重写基因组序列是基因组编辑的研究热点，也是推动工业生物技术快速发展的核心使能技术。基因组编辑技术经历了三个发展阶段，从锌指核酸酶（ZFNs），到转录激活样效应物核酸酶（TALEN）和Cas核酸酶。目前，CRISPR/Cas的蓬勃发展使研究人员能够建立一系列基于第一代和第二代Cas的基因组编辑技术。这有助于建立和优化原核底盘细胞（如 ）或真核底盘细胞（如 ）。本文总结了利用传统底盘细胞的工业生物技术的当前发展和应用，并展望了未来的发展趋势，旨在帮助研究人员优化工业生物技术及其潜在应用。
