Lambert M
Eur J Cancer Clin Oncol. 1986 Jul;22(7):781-5. doi: 10.1016/0277-5379(86)90363-9.
Tritiated thymidine labelling indices (TLIs) were determined on a number of primary cancers of the human breast. Twenty-two slides were chosen which demonstrated a wide range of TLIs and each was counted twice to assess 'counting error'. TLIs derived from successive counts of the same slide showed a coefficient of variance greater than 25% in half of the slides, but a significant difference between the two (P less than 0.05) in only 2 of 22 pairs. When TLIs derived from paired specimens taken from different sites in each of 22 tumours were compared, there was found to be a significant difference (P less than 0.05) in 17 of 22 pairs. This is 'sampling error' and is clearly a major source of inaccuracy when TLIs are derived from single small samples of heterogeneous tumours such as cancers of the human breast.
测定了许多人类原发性乳腺癌的氚标记胸腺嘧啶核苷标记指数（TLIs）。选择了22张显示出广泛TLIs范围的切片，每张切片计数两次以评估“计数误差”。来自同一切片连续计数的TLIs在一半的切片中显示变异系数大于25%，但在22对中只有2对两者之间存在显著差异（P小于0.05）。当比较从22个肿瘤中每个肿瘤不同部位采集的配对标本得出的TLIs时，发现22对中有17对存在显著差异（P小于0.05）。这就是“抽样误差”，当从异质性肿瘤（如人类乳腺癌）的单个小样本得出TLIs时，显然是不准确的一个主要来源。
