Madaan Priyanka, Sharma Uttam, Tyagi Nipanshi, Brar Balvinder Kaur, Bansal Shivani, Kushwaha Hemant Rituraj, Kapoor Harmanpreet Singh, Jain Aklank, Jain Manju
Department of Biochemistry, Central University of Punjab, Bathinda, Punjab, India.
Department of Zoology, Central University of Punjab, Bathinda, Punjab, India.
Front Med (Lausanne). 2023 Aug 28;10:1207993. doi: 10.3389/fmed.2023.1207993. eCollection 2023.
Psoriasis is a chronic inflammatory skin disease with keratinocyte hyperproliferation and T cells as key mediators of lesional and systemic inflammatory changes. To date, no suitable differential biomarkers are available for the disease diagnosis. More recently, microRNAs have been identified as critical regulators of lesional and systemic immune changes in psoriasis with diagnostic potential. We have performed expression profiling of T cell-specific miRNAs in 38 plasma samples from psoriasis vulgaris patients and an equal number of age- and gender-matched healthy subjects. Our findings have identified a panel of five blood-based circulatory miRNAs with a significant change in their expression levels, comprising miR-215, miR-148a, miR-125b-5p, miR-223, and miR-142-3p, which can differentiate psoriasis vulgaris patients from healthy individuals. The receiver operating characteristic (ROC) curves for all five miRNAs individually and in combination exhibited a significant disease discriminatory area under the curve with an AUC of 0.762 and a < 0.0001 for all the miRNAs together. Statistically, all five miRNAs in combination depicted the best-fit model in relation to disease severity (PASI) compared with individual miRNAs, with the highest R value of 0.94 and the lowest AIC score of 131.8. Each of the miRNAs also exhibited a significant association with at least one of the other miRNAs in the panel. Importantly, the five miRNAs in the panel regulate one or more immune-inflammation pathways based on target prediction, pathway network analysis, and validated roles in the literature. The miRNA panel provides a rationalized combination of biomarkers that can be tested further on an expanded cohort of patients for their diagnostic value.
银屑病是一种慢性炎症性皮肤病，角质形成细胞过度增殖，T细胞是皮损和全身炎症变化的关键介质。迄今为止，尚无合适的鉴别生物标志物可用于该疾病的诊断。最近，微小RNA已被确定为银屑病皮损和全身免疫变化的关键调节因子，具有诊断潜力。我们对38份寻常型银屑病患者的血浆样本以及相同数量的年龄和性别匹配的健康受试者进行了T细胞特异性微小RNA的表达谱分析。我们的研究结果确定了一组五个基于血液的循环微小RNA，它们的表达水平有显著变化，包括miR-215、miR-148a、miR-125b-5p、miR-223和miR-142-3p，这些微小RNA可以区分寻常型银屑病患者和健康个体。所有五个微小RNA单独及联合的受试者工作特征（ROC）曲线均显示曲线下有显著的疾病鉴别区域，所有微小RNA联合时的曲线下面积（AUC）为0.762，P<0.0001。统计学上，与单个微小RNA相比，所有五个微小RNA联合描绘了与疾病严重程度（银屑病面积和严重程度指数，PASI）最拟合的模型，最高R值为0.94，最低赤池信息准则（AIC）评分为131.8。每个微小RNA也与该组中的至少一个其他微小RNA表现出显著关联。重要的是，根据靶标预测、通路网络分析以及文献中已证实的作用，该组中的五个微小RNA调节一种或多种免疫炎症通路。该微小RNA组合提供了一种合理的生物标志物组合，可在更大规模的患者队列中进一步测试其诊断价值。
