Das Moumalini, Banerjee Abhishek, Samanta Jaydeep, Bhunia Banga Bhusan, Mozumder Samaresh, Ramalingam Karthikeyan
Oral and Maxillofacial Pathology, Awadh Dental College and Hospital, Jamshedpur, IND.
Orthodontics and Dentofacial Orthopedics, Haldia Institute of Dental Sciences and Research, Haldia, IND.
Cureus. 2023 Aug 12;15(8):e43386. doi: 10.7759/cureus.43386. eCollection 2023 Aug.
Introduction Collection of blood samples from mass disaster victims and blood grouping is a challenging task. This can be attributed to various reasons. From the forensic odontology perspective, teeth and bones are one of the noteworthy remains that can be derived from such sites. Aims and objectives The aim of our study is to detect ABO blood groups and Rhesus (Rh) factor from extirpated pulp tissue of the extracted teeth at zero, three, and six months' time interval by absorption-elution technique. Materials and methods The study consisted of 90 freshly extracted teeth as suggested by a biostatistician. Thirty teeth were analyzed immediately and 60 teeth were stored in vials without any preservative at room temperature. The pulp tissue was extirpated and studied at zero months, three months, and six months to determine blood groups and Rh factors. The extraction socket blood was tested to identify the blood group of that patient and used as a control reference. The blood grouping was done at respective time periods through the absorption-elution method and matched with the control. Data were analyzed using Statistical Package for the Social Sciences (SPSS) version 24 software (IBM Corp., Armonk, NY). The chi-square test and Kruskal-Wallis test were done. A p-value < 0.05 was considered statistically significant. Results Pulp showed the highest sensitivity for blood groups at zero months but it could be identified up to six months, although the sensitivity and specificity gradually decreased. ABO blood grouping showed higher sensitivity than the Rh factor as time progressed. Conclusion In cases where teeth are the only remains in a forensic condition, the dental pulp can be an authentic source for blood group detection.
引言 从大规模灾难受害者身上采集血样并进行血型鉴定是一项具有挑战性的任务。这可归因于多种原因。从法医牙科学的角度来看，牙齿和骨骼是此类现场值得注意的遗留物之一。
目的 我们研究的目的是通过吸收-洗脱技术，在零个月、三个月和六个月的时间间隔从拔除牙齿的牙髓组织中检测ABO血型和恒河猴（Rh）因子。
材料和方法 按照生物统计学家的建议，该研究包括90颗新鲜拔除的牙齿。立即对30颗牙齿进行分析，60颗牙齿在室温下不添加任何防腐剂的小瓶中保存。在零个月、三个月和六个月时对牙髓组织进行摘除并研究，以确定血型和Rh因子。对拔牙窝血液进行检测以确定该患者的血型，并用作对照参考。在各个时间段通过吸收-洗脱法进行血型鉴定，并与对照进行匹配。使用社会科学统计软件包（SPSS）24版软件（IBM公司，纽约州阿蒙克）对数据进行分析。进行卡方检验和克鲁斯卡尔-沃利斯检验。p值<0.05被认为具有统计学意义。
结果 牙髓在零个月时对血型的敏感性最高，但尽管敏感性和特异性逐渐降低，仍可在六个月内进行鉴定。随着时间的推移，ABO血型鉴定的敏感性高于Rh因子。
结论 在法医情况下牙齿是唯一遗留物的案例中，牙髓可以是血型检测的可靠来源。
