Rajawat Gaurav, Ramalingam Karthikeyan, Pareek Rajat, Singh Gagandeep, Narula Harleen, Aggarwal Atul
Oral Pathology and Microbiology, Surendera Dental College and Research Institute, Sriganganagar, IND.
Oral pathology, Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Chennai, IND.
Cureus. 2023 Apr 11;15(4):e37415. doi: 10.7759/cureus.37415. eCollection 2023 Apr.
Aim To estimate the ABO blood groups from saliva samples and to correlate with the secretor status. Materials and methods A sample size of 300 individuals was selected from the outpatient department of Surendera Dental College & Research Institute, Sriganganagar, India, and from dental camps organized by the college in the near vicinity. Informed consent was obtained from selected individuals to collect their blood and saliva samples. Salivary samples were evaluated for ABO blood groups by the absorption-inhibition method. The indicator erythrocytes were prepared after blood group confirmation from serum. It was used to identify the blood group antigens in saliva to confirm the secretor status. The results were tabulated and the Pearson's chi-squared test was performed for statistical analysis using SPSS 15.0 (SPSS Inc., Chicago, IL). Results The present study showed that 282 subjects (94%) were Rhesus positive and 18 subjects (6%) were Rhesus negative. Two-hundred-and-fifty subjects (83.3%) were secretors of antigens in saliva. Non-secretors were 50 subjects (16.7%). We identified that 250/300 were secretors and the majority were in AB & A group. Conclusion Blood groups could not be detected from the saliva of subjects who were non-secretors. In contrast, blood types could be accurately identified from the saliva of those subjects who were secretors of antigen.
目的 从唾液样本中估计ABO血型，并与分泌型状态相关联。材料与方法 从印度斯利甘加纳格尔市苏伦德拉牙科学院及研究所门诊部以及该学院在附近组织的牙科义诊活动中选取300名个体作为样本。获得选定个体的知情同意后，采集他们的血液和唾液样本。通过吸收抑制法对唾液样本进行ABO血型评估。在从血清确认血型后制备指示红细胞。用其鉴定唾液中的血型抗原以确认分泌型状态。将结果制成表格，并使用SPSS 15.0（SPSS公司，伊利诺伊州芝加哥）进行Pearson卡方检验以进行统计分析。结果 本研究表明，282名受试者（94%）为Rh阳性，18名受试者（6%）为Rh阴性。250名受试者（83.3%）是唾液中抗原的分泌者。非分泌者有50名受试者（16.7%）。我们确定300人中250人为分泌者，且大多数在AB型和A型组。结论 非分泌型受试者的唾液中无法检测到血型。相比之下，抗原分泌型受试者的唾液中可以准确鉴定出血型。
