Xingzhi X, Ji L, Hao F, Ming L, Zhuyao L
Department of Forensic Medicine, Shanghai Medical University, China.
J Forensic Sci. 1993 Jul;38(4):956-60.
Twenty-five permanent teeth, including eight carious ones whose pulp cavities had been exposed, were used for this research 3-5 weeks after extraction. Phosphate-buffered saline (PBS, at pH 7.2) was employed to extract ABO blood group substance from tooth powder. ABO grouping was performed on blood-stained compresses from the extraction wound (as controls), tooth fragment, tooth powder, and cotton fibers immersed in PBS extract by absorption-elution (AE) technique and on the PBS extracts by the two-dimensional absorption-inhibition (2-D AI) technique. It was found that blood grouping in PBS extracts by 2-D AI yielded reliable results: no false positive results, and a high rate of correct grouping, (24/25), while blood grouping on other dental materials, such as tooth fragments, tooth powders, immersed fibers, by AE gave an unacceptable rate of false positive/negative results.
本研究使用了25颗恒牙，其中包括8颗牙髓腔已暴露的龋齿，这些牙齿在拔除后3 - 5周用于实验。采用磷酸盐缓冲盐水（PBS，pH 7.2）从牙粉中提取ABO血型物质。通过吸收 - 洗脱（AE）技术对拔牙创口的血渍敷料（作为对照）、牙碎片、牙粉以及浸泡在PBS提取物中的棉纤维进行ABO血型分组，并通过二维吸收抑制（2 - D AI）技术对PBS提取物进行血型分组。结果发现，通过2 - D AI技术对PBS提取物进行血型分组可得到可靠结果：无假阳性结果，且正确分组率高（24/25），而通过AE技术对其他牙科材料（如牙碎片、牙粉、浸泡纤维）进行血型分组时，假阳性/阴性结果的发生率令人无法接受。
