Abdallah Rokia M, Hammoda Hala M, El-Gazzar Nahla S, Ibrahim Reham S, Sallam Shaimaa M
Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University Alexandria Egypt
RSC Adv. 2023 Sep 11;13(39):27167-27173. doi: 10.1039/d3ra05826c. eCollection 2023 Sep 8.
Activity-guided fractionation of the ethanolic extracts of and furnished eight compounds with pancreatic lipase inhibitory activity. Six compounds were isolated from the chloroform fraction of . It is worth mentioning that this is the first report for the isolation of 5,7,4'-trihydroxy-8-methoxycarbonyl flavanol (2), daphnodorin G-3''-methyl ether (4) and daphnodorin G (5) from genus . Moreover, daphnoretin (1), neochamaejasmin A (3) and daphnodorin B (6) were also isolated from the chloroform fraction of the same plant. On the other hand, quercetin 3--α-l-rhamnopyranosyl-(1 → 2)-[α-l-rhamnopyranosyl-(1 → 6)]-β-d-galactopyranoside (7) and 3--[α-l-fucopyranosyl-(1 → 2)-β-d-glucopyranosyl-(1 → 3)-α-l-arabinopyranosyl] jujubogenin (christinin A) (8) were isolated from the -butanol fraction of . Structure elucidation of the isolated compounds was carried out by detailed analysis of 1D and 2D spectral data. These compounds showed percentage inhibitions of 72% (1), 52% (2), 61.8% (3), 39% (4), 69.5% (5), 3.5% (6), 68% (7) and 75% (8) at the concentration of 250 μM and XP-G scores of lipase inhibition were 11.40 (1), 8.71 (2), 6.13 (3), 8.23 (4), 6.22 (5), 9.76 (6), 14.66 (7) and 12.00 (8). This is the first report of the isolation of lipase inhibitors from both plants and . In addition to that, this might result in presenting the biscoumarin, daphnoretin, and the dammarane saponin, christinin A, as potent lipase inhibitors.
对[植物名称1]和[植物名称2]乙醇提取物进行活性导向分离,得到了8种具有胰脂肪酶抑制活性的化合物。从[植物名称1]的氯仿部位分离出6种化合物。值得一提的是,这是首次从[植物属名]中分离出5,7,4'-三羟基-8-甲氧基羰基黄烷醇(2)、瑞香毒素G - 3'' - 甲醚(4)和瑞香毒素G(5)。此外,瑞香素(1)、新白花丹素A(3)和瑞香毒素B(6)也从同一植物的氯仿部位分离得到。另一方面,槲皮素3 - [α - l - 鼠李糖基 - (1→2) - [α - l - 鼠李糖基 - (1→6)] - β - d - 半乳糖苷(7)和3 - [α - l - 岩藻糖基 - (1→2) - β - d - 葡萄糖基 - (1→3) - α - l - 阿拉伯糖基]枣皂苷元(克里斯汀宁A)(8)从[植物名称1]的正丁醇部位分离得到。通过对一维和二维光谱数据的详细分析对分离得到的化合物进行结构解析。这些化合物在250μM浓度下的抑制率分别为72%(1)、52%(2)、61.8%(3)、39%(4)、69.5%(5)、3.5%(6)、68%(7)和75%(8),脂肪酶抑制的XP - G评分分别为11.40(1)、8.71(2)、6.13(3)、8.23(4)、6.22(5)、9.76(6)、14.66(7)和12.00(8)。这是首次从[植物名称1]和[植物名称2]中分离出脂肪酶抑制剂的报道。除此之外,这可能使得双香豆素、瑞香素以及达玛烷皂苷克里斯汀宁A成为有效的脂肪酶抑制剂。