来自枣椰树叶子的达玛烷型皂苷。
Dammarane-type saponins from leaves of Ziziphus spina-christi.
作者信息
Bozicevic Alen, De Mieri Maria, Di Benedetto Angela, Gafner Frank, Hamburger Matthias
机构信息
Institute of Pharmaceutical Biology, University of Basel, Klingelbergstrasse 50, CH-4056, Basel, Switzerland.
Mibelle Biochemistry, Mibelle AG, 5033, Buchs, Switzerland.
出版信息
Phytochemistry. 2017 Jun;138:134-144. doi: 10.1016/j.phytochem.2017.02.028. Epub 2017 Mar 6.
Phytochemical profiling of Ziziphus spina-christi leaves led to the characterization of 10 dammarane-type saponins and 12 phenolic compounds. Isolation was achieved by gel chromatography on Sephadex LH20, open column chromatography on silica gel, and semi-preparative HPLC with PDA and ELSD detectors. Structural characterization was performed by extensive 1D and 2D NMR, mass spectrometry, and by GC-MS of sugar derivatives. A biosynthetic pathway leading to three previously undescribed sapogenins is proposed. The saponin profiles in Z. spina-christi leaves of four different origins were compared by means of HPLC-ESIMS.
对枣树叶进行植物化学分析，鉴定出10种达玛烷型皂苷和12种酚类化合物。通过Sephadex LH20凝胶色谱、硅胶柱色谱以及配备PDA和ELSD检测器的半制备HPLC进行分离。通过广泛的一维和二维核磁共振、质谱以及糖衍生物的气相色谱-质谱联用对结构进行表征。提出了一条通往三种此前未描述的皂苷元的生物合成途径。通过HPLC-ESIMS比较了四个不同产地的枣树叶中的皂苷谱。
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