Study of IsCT analogue peptide against and toxicity/teratogenicity in zebrafish embryos ().

An IsCT analogue peptide (PepM3) was designed based on structural studies of wasp mastoparans and tested against . Its effects on fungal cell membranes and toxicity were evaluated. Antifungal activity was analyzed using a microdilution susceptibility test. Toxicity was assessed using human skin keratinocytes (HaCaT) and zebrafish embryos. PepM3 demonstrated activity against and a synergistic effect with amphotericin B. The peptide presented fungicidal action with damage to the fungal cell membrane, low toxicity in HaCat cells and was nonteratogenic in zebrafish embryos. Evaluating structural modifications is essential for the development of new agents with potential activity against fungal pathogens and for the reduction of toxic and teratogenic effects.