Aminov Ruslan, Frolov Alexander, Aminova Alina
Department of Biology, Faculty of Biology, Zaporizhzhia National University, Zhukovsky Street, 66, 69600 Ukraine.
Ann Parasitol. 2022;68(4):721-726. doi: 10.17420/ap6804.479.
Ectoparasitic leeches have many biologically active substances in their body, which are able to show various therapeutic effects, which makes them very relevant in the study. Among them, the most common are medicinal leeches: Hirudo verbana, Hirudo medicinalis and Hirudo orientalis. They are listed in the Red Book as a vulnerable species, so their population is mostly supported in biolabs. Therefore, the search for different methods of their preservation is relevant. The aim of the work was to test the effect of the duration of rest and feeding of animals for re- reproduction, which will increase their population. For the study, three experimental animal groups were formed: 1 control - the animals were fed a week after the first reproduction, re-reproduction occurred not earlier than 2 months; 2 experimental - selected animals that remained clitellum after the first reproduction and again sent to the peat-soil environment for reproduction without rest and feeding; 3 experimental - selected animals that remained clitellum after the first reproduction, fed after 1 week, after another 1-2 weeks again sent to the peat-soil medium for reproduction. As a result of the study, the restoration of the physiological state after the first dilution in the first and third groups was registered. In the second group, the animals are depleted as a result of defective offspring, mortality of them and their offspring.
体外寄生水蛭体内含有多种生物活性物质，能够展现出各种治疗效果，这使得它们在研究中具有很高的相关性。其中，最常见的药用蛭类有：欧洲医蛭、医用蛭和东方医蛭。它们被列入《红皮书》为易危物种，所以其种群大多在生物实验室中得到维持。因此，寻找不同的保存方法具有现实意义。该研究的目的是测试动物繁殖前休息和喂食时长的影响，这将增加它们的种群数量。为进行该研究，组建了三个实验动物组：1. 对照组——动物在首次繁殖一周后喂食，再次繁殖不早于2个月；2. 实验组1——选取首次繁殖后仍保留环带的动物，不休息、不喂食，再次放入泥炭土环境中繁殖；3. 实验组2——选取首次繁殖后仍保留环带的动物，1周后喂食，再过1 - 2周后再次放入泥炭土介质中繁殖。研究结果显示，第一组和第三组在首次繁殖后生理状态得以恢复。在第二组中，由于后代缺陷、动物及其后代的死亡，动物变得衰弱。
