miR-638 通过抑制 在汉防己甲素治疗下的表达来抑制宫颈癌的进展。
miR-638 suppresses cervical cancer progression by inhibiting under the treatment of Tetrandrine.
机构信息
Department of Gynecology, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, Hubei, PR China.
Department of Nuclear Medicine, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, Hubei, PR China.
出版信息
Histol Histopathol. 2024 Apr;39(4):497-509. doi: 10.14670/HH-18-657. Epub 2023 Aug 4.
BACKGROUND
The interaction of microRNA with Chinese herbal medicines is a promising therapeutic approach for prevention of cervical cancer.
METHODS
Western blotting or qRT-PCR were carried out to identify the expression of and miR-638. A tetrandrine (TET) cell model was used to explore the effects of miR-638 and its target gene using CCK-8, transwell, wound healing, and western blot assays. Furthermore, luciferase activity assay was conducted to measure the interaction among TET, and miR-638.
RESULTS
Under TET treatment, Hela and SiHa cells exhibited repressed cell viability, migration, invasion, and epithelial-mesenchymal transition (EMT), and these effects were further enhanced by high expression of miR-638. In contrast, expression was low in TET-treated cells and had an opposite effect to that of miR-638.
CONCLUSION
We highlighted that miR-638 suppresses cervical cancer progression by inhibiting under tetrandrine treatment.
背景
miRNA 与中草药的相互作用是预防宫颈癌的一种有前途的治疗方法。
方法
采用 Western blot 或 qRT-PCR 鉴定 和 miR-638 的表达。用汉防己甲素（TET）细胞模型，采用 CCK-8、Transwell、划痕愈合和 Western blot 实验，探讨 miR-638 及其靶基因 的作用。此外，通过荧光素酶活性测定来测量 TET、 和 miR-638 之间的相互作用。
结果
TET 处理后，Hela 和 SiHa 细胞的细胞活力、迁移、侵袭和上皮间质转化（EMT）受到抑制，而 miR-638 的高表达进一步增强了这些作用。相反，在 TET 处理的细胞中 的表达较低，其作用与 miR-638 相反。
结论
我们强调 miR-638 通过抑制 tetrandrine 处理下的 来抑制宫颈癌的进展。
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