General Medicine, Harrison International Peace Hospital, Hengshui, Hebei province, China.
Department of Anesthesiology, Harrison International Peace Hospital, Hengshui, Hebei province, China.
Cell Biol Int. 2018 Sep;42(9):1115-1123. doi: 10.1002/cbin.10974. Epub 2018 Jul 11.
MicroRNAs (miRNAs) play key roles in cervical cancer metastasis progression. Accumulated evidences have revealed that miRNAs are related to the pathophysiological process. However, the role of miR-340 in cervical cancer and how it works is still not fully interpreted. Using qRT-PCR to examine the expression of miR-340 in cervical cancer tissues. Transwell migration and invasion experiments were used to detect the role of miR-340 in migration and invasion. Luciferase reporter assay, qRT-PCR, and Western blot were used to detect the relationship between miR-340 and EphA3. Using Transwell migration and invasion experiments to investigate the role of EphA3 on migration and invasion. Restoration expriments were also performed. Western blot was used to assay the influence of miR-340 and EphA3 on EMT. We found that miR-340 was downregulated in cervical cancer tissues compared with the normal tissues. Transwell migration and invasion experiments indicated that overexpression of miR-340 frequently inhibited the migration and invasion of cervical cancer cells. EphA3 is a target of miR-340, and ectopic expression of EphA3 can promote the migration and invasion of cervical cancer cells, whereas restoration of EphA3 in miR-340-overexpressing cervical cancer cells reversed the suppressive effects of miR-340. What's more, the process of migration and invasion which regulated by miR-340/EphA3 was depended on adjusting the EMT way. These findings indicate that miR-340 may act as an anti-tumor factor during the process of tumor metastasis through targeting EphA3, suggesting that miR-340 might be a potential new diagnostic and therapeutic molecule for the treatment of cervical cancer.
微小 RNA（miRNAs）在宫颈癌转移进展中发挥关键作用。越来越多的证据表明，miRNAs 与病理生理过程有关。然而，miR-340 在宫颈癌中的作用及其作用机制仍未完全阐明。本研究采用 qRT-PCR 检测宫颈癌组织中 miR-340 的表达。通过 Transwell 迁移和侵袭实验检测 miR-340 对迁移和侵袭的作用。荧光素酶报告实验、qRT-PCR 和 Western blot 检测 miR-340 与 EphA3 之间的关系。通过 Transwell 迁移和侵袭实验研究 EphA3 对迁移和侵袭的作用。还进行了恢复实验。Western blot 用于检测 miR-340 和 EphA3 对 EMT 的影响。结果发现，与正常组织相比，宫颈癌组织中 miR-340 表达下调。Transwell 迁移和侵袭实验表明，miR-340 的过表达常抑制宫颈癌细胞的迁移和侵袭。EphA3 是 miR-340 的靶基因，EphA3 的异位表达可促进宫颈癌细胞的迁移和侵袭，而在 miR-340 过表达的宫颈癌细胞中恢复 EphA3 可逆转 miR-340 的抑制作用。此外，miR-340/EphA3 调节的迁移和侵袭过程依赖于 EMT 方式的调节。这些发现表明，miR-340 通过靶向 EphA3 可能在肿瘤转移过程中发挥抑癌因子的作用，提示 miR-340 可能成为治疗宫颈癌的潜在新的诊断和治疗分子。
