Winklbauer R
Dev Biol. 1986 Nov;118(1):70-81. doi: 10.1016/0012-1606(86)90074-6.
The requirements for cell division in ectodermal blastomeres of the early Xenopus embryo were studied. Isolated blastomeres divide autonomously on nonadhesive agar in a simple salt solution up to the midblastula stage. After the midblastula transition, cell-cell contact is required for blastomere division. In isolated blastomeres of that stage, cytokinesis fails, but nuclear division continues normally for some time. Cell-cell contact as a prerequisite for blastomere division can be replaced by culturing blastomeres on an appropriate substratum. Clonal growth of isolated blastomeres is supported by a variety of protein substrata, indicating rather unspecific substratum requirements. Different substrata which do not support blastomere division can affect different steps in cytokinesis.
研究了非洲爪蟾早期胚胎外胚层卵裂球的细胞分裂需求。分离的卵裂球在简单盐溶液中的非粘性琼脂上自主分裂，直至囊胚中期。囊胚中期转变后，卵裂球分裂需要细胞间接触。在该阶段的分离卵裂球中，胞质分裂失败，但核分裂会正常持续一段时间。作为卵裂球分裂前提条件的细胞间接触，可以通过在合适的基质上培养卵裂球来替代。多种蛋白质基质支持分离卵裂球的克隆生长，表明对基质的要求相当不特异。不支持卵裂球分裂的不同基质会影响胞质分裂的不同步骤。
