Jacobs R E, Fraser S E
Division of Biology, Beckman Institute, California Institute of Technology, Pasadena 91125.
Science. 1994 Feb 4;263(5147):681-4. doi: 10.1126/science.7508143.
Key events in vertebrate embryogenesis are difficult to observe in many species. High-resolution magnetic resonance imaging was used to follow cell movements and lineages in developing frog embryos. A single cell was injected at the 16-cell stage with a contrast agent, based on the gadolinium chelate gadolinium-diethylenetriamine pentaacetic acid-dextran. The labeled progeny cells could be followed uniquely in three-dimensional magnetic resonance images, acquired from the embryo over several days. The results show that external ectodermal and internal mesodermal tissues extend at different rates during amphibian gastrulation and neurulation.
在许多物种中，脊椎动物胚胎发育过程中的关键事件很难被观察到。高分辨率磁共振成像被用于追踪发育中的青蛙胚胎中的细胞运动和谱系。在16细胞阶段，向单个细胞注射了一种基于钆螯合物钆-二乙烯三胺五乙酸-葡聚糖的造影剂。标记的后代细胞可以在从胚胎上采集的三维磁共振图像中被唯一追踪，这些图像是在几天内获取的。结果表明，在两栖动物原肠胚形成和神经胚形成过程中，外部外胚层和内部中胚层组织以不同的速率延伸。
