Hu Yaqi, Wang Rui, Wang Shuwen, Yuan Chengfu, Yuan Qi, Zhang Yifan, Ren Jianxia, He Yumin, Wu Xiaoshan, Dai Wenjun, Wang Wenyong, Xie Shijun
College of Basic Medical Science, China Three Gorges University.
Hubei Provincial Clinical Research Center for Precise Prevention and Treatment of Elderly Gastrointestinal Cancer, The Second People's Hospital of China Three Gorges University.
J Oleo Sci. 2023 Oct 3;72(10):929-938. doi: 10.5650/jos.ess23065. Epub 2023 Sep 12.
Selenium-enriched polysaccharides from Pyracantha fortuneana (SePFP) has many beneficial physiological activities, but how it improves the aging associated abnormal lipid metabolism is still unclear. Therefore, we explored the mechanisms of the regulatory role of SePFP on liver lipid accumulation in aging mice.
60 naturally aged C57BL/6J male mice were divided into 6 groups: adult group, aging group (21-month-old mice), aging mice treated with low-, medium- and high-doses of SePFP (SePFP-L, SePFP-M, SePFP-H), and aging mice treated with resveratrol (RSV). SePFP and RSV were administrated daily via oral gavage from 16 to 21 months old. The parameters of energy metabolism were measured in all mice before sacrifice, and liver tissues were collected to determine the levels of metabolism-related enzymes by real-time PCR and Western blot.
We found that SePFP significantly reduced the body weight, liver to bodyweight ratio, and white fat to body weight ratio in aging mice. SePFP also down-regulated the triglycerides and cholesterol levels in liver and serum, and decreased respiratory quotient in aging mice. The mechanism of SePFP regulating lipid metabolism was mainly through promoting fatty acid transportation to mitochondria and enhancing mitochondrial β-oxidation and ketone body production.
SePFP attenuates liver lipid deposition in aging mice by enhancing hepatic mitochondrial β-oxidation.
火棘富硒多糖（SePFP）具有多种有益的生理活性，但其如何改善与衰老相关的异常脂质代谢仍不清楚。因此，我们探讨了SePFP对衰老小鼠肝脏脂质积累的调节作用机制。
将60只自然衰老的C57BL/6J雄性小鼠分为6组：成年组、衰老组（21月龄小鼠）、低、中、高剂量SePFP处理的衰老小鼠（SePFP-L、SePFP-M、SePFP-H）以及白藜芦醇（RSV）处理的衰老小鼠。从16至21月龄开始，每天通过灌胃给予SePFP和RSV。在处死所有小鼠前测量能量代谢参数，并收集肝脏组织通过实时PCR和蛋白质印迹法测定代谢相关酶的水平。
我们发现SePFP显著降低了衰老小鼠的体重、肝脏与体重比以及白色脂肪与体重比。SePFP还下调了衰老小鼠肝脏和血清中的甘油三酯和胆固醇水平，并降低了呼吸商。SePFP调节脂质代谢的机制主要是通过促进脂肪酸向线粒体的转运以及增强线粒体β氧化和酮体生成。
SePFP通过增强肝脏线粒体β氧化减轻衰老小鼠的肝脏脂质沉积。
