Graduate Institute of Oral Biology, College of Medicine, National Taiwan University, Taipei, Taiwan.
Graduate Institute of Oral Biology, College of Medicine, National Taiwan University, Taipei, Taiwan; School of Dentistry, National Taiwan University, Taipei, Taiwan; Graduate Institute of Clinical Dentistry, School of Dentistry, National Taiwan University, Taipei, Taiwan; Department of Dentistry, National Taiwan University Hospital, College of Medicine, Taipei, Taiwan.
J Formos Med Assoc. 2024 Mar;123(3):390-399. doi: 10.1016/j.jfma.2023.08.020. Epub 2023 Sep 11.
BACKGROUND/PURPOSE: Fibroblast growth factor (FGF) 5 is a member of the FGF family that functions as a regulator of tissue growth and regeneration. Aberrant FGF5 expression has been previously associated with the progression of a number of different malignancies. However, its potential role in oral cancer remains unclear. In this study, we explored the relationship between the expression of FGF5 protein in oral squamous cell carcinomas (OSCCs) and the clinicopathological parameters of OSCCs and whether the expression of FGF5 protein in OSCCs could be a prognostic factor for OSCC patients.
The FGF5 protein expression was examined in 64 OSCC and 34 normal oral mucosal specimens by immunohistochemical staining. Stress induced upregulation and intracellular redistribution of FGF5 were verified using xenograft animal model and OSCC cell lines.
The mean FGF5 protein labelling index was significantly higher in OSCC than in normal oral mucosal samples, with high FGF5 protein labelling index (>58%) being correlated with advanced stage and poor survival of OSCC patients. Apart from the peri-cytoplasmic staining pattern characteristic of paracrine growth factors, FGF5 protein was localized as distinct punctate structures in the cytoplasm of advanced stage or stressed-induced cells. This redistribution and upregulation of FGF5 protein could be sustained after termination of the stress induction in cell line and xenograft animal models.
FGF5 can be induced by cellular stress and risk factors of OSCC, where high expression levels of FGF5 is potentially a useful parameter for predicting OSCC progression and patient survival.
背景/目的：成纤维细胞生长因子 5（FGF5）是 FGF 家族的一员，作为组织生长和再生的调节剂发挥作用。异常的 FGF5 表达先前与多种不同恶性肿瘤的进展有关。然而，其在口腔癌中的潜在作用尚不清楚。在这项研究中，我们探讨了 FGF5 蛋白在口腔鳞状细胞癌（OSCC）中的表达与 OSCC 的临床病理参数之间的关系，以及 OSCC 中 FGF5 蛋白的表达是否可以作为 OSCC 患者的预后因素。
通过免疫组织化学染色检测 64 例 OSCC 和 34 例正常口腔黏膜标本中的 FGF5 蛋白表达。使用异种移植动物模型和 OSCC 细胞系验证 FGF5 蛋白的应激诱导上调和细胞内重新分布。
OSCC 中的 FGF5 蛋白标记指数明显高于正常口腔黏膜样本，高 FGF5 蛋白标记指数（>58%）与 OSCC 患者的晚期阶段和较差的生存相关。除了旁分泌生长因子特有的胞周染色模式外，FGF5 蛋白还定位于晚期或应激诱导细胞的细胞质中明显的点状结构。这种 FGF5 蛋白的重新分布和上调可以在细胞系和异种移植动物模型中应激诱导终止后持续存在。
FGF5 可被细胞应激和 OSCC 的危险因素诱导，FGF5 高表达可能是预测 OSCC 进展和患者生存的有用参数。
