Regulation of Hair Follicle Growth and Development by Different Alternative Spliceosomes of FGF5 in Rabbits.

This study investigated the regulatory effect of alternative spliceosomes of the fibroblast growth factor 5 () gene on hair follicle (HF) growth and development in rabbits. The FGF5 alternative spliceosomes (called , , ) were cloned. The overexpression vector and siRNA of spliceosomes were transfected into dermal papilla cells (DPCs) to analyze the regulatory effect on DPCs. The results revealed that and overexpression significantly decreased mRNA expression ( < 0.01). overexpression significantly reduced expression ( < 0.01). and possibly downregulated the expression level of mRNA ( < 0.05), and FGF5-X3 significantly downregulated the expression level of mRNA ( < 0.01). The FGF5 alternative spliceosomes significantly downregulated the mRNA expression level in both cases ( < 0.01). and significantly increased mRNA expression ( < 0.01). All three FGF5 alternative spliceosomes inhibited DPC proliferation. In conclusion, the expression profile of HF growth and development-related genes can be regulated by FGF5 alternative spliceosomes, inhibiting the proliferation of DPCs and has an influence on the regulation of HF growth in rabbits. This study provides insights to further investigate the mechanism of HF development in rabbits via regulation.