Sun Shaoning, Zhao Bohao, Li Jiali, Zhang Xiyu, Yao Shuyu, Bao Zhiyuan, Cai Jiawei, Yang Jie, Chen Yang, Wu Xinsheng
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Joint International Research Laboratory of Agriculture & Agri-Product Safety, Yangzhou University, Yangzhou 225009, China.
Genes (Basel). 2024 Mar 26;15(4):409. doi: 10.3390/genes15040409.
This study investigated the regulatory effect of alternative spliceosomes of the fibroblast growth factor 5 () gene on hair follicle (HF) growth and development in rabbits. The FGF5 alternative spliceosomes (called , , ) were cloned. The overexpression vector and siRNA of spliceosomes were transfected into dermal papilla cells (DPCs) to analyze the regulatory effect on DPCs. The results revealed that and overexpression significantly decreased mRNA expression ( < 0.01). overexpression significantly reduced expression ( < 0.01). and possibly downregulated the expression level of mRNA ( < 0.05), and FGF5-X3 significantly downregulated the expression level of mRNA ( < 0.01). The FGF5 alternative spliceosomes significantly downregulated the mRNA expression level in both cases ( < 0.01). and significantly increased mRNA expression ( < 0.01). All three FGF5 alternative spliceosomes inhibited DPC proliferation. In conclusion, the expression profile of HF growth and development-related genes can be regulated by FGF5 alternative spliceosomes, inhibiting the proliferation of DPCs and has an influence on the regulation of HF growth in rabbits. This study provides insights to further investigate the mechanism of HF development in rabbits via regulation.
本研究探讨了成纤维细胞生长因子5（FGF5）基因可变剪接体对家兔毛囊（HF）生长发育的调控作用。克隆了FGF5可变剪接体（分别称为FGF5 - X1、FGF5 - X2、FGF5 - X3）。将剪接体的过表达载体和小干扰RNA（siRNA）转染至真皮乳头细胞（DPCs）中，以分析其对DPCs的调控作用。结果显示，FGF5 - X1和FGF5 - X2过表达显著降低FGF5 mRNA表达（P < 0.01）。FGF5 - X3过表达显著降低FGF5表达（P < 0.01）。FGF5 - X1和FGF5 - X2可能下调FGF5 mRNA的表达水平（P < 0.05），且FGF5 - X3显著下调FGF5 mRNA的表达水平（P < 0.01）。在两种情况下，FGF5可变剪接体均显著下调FGF5 mRNA表达水平（P < 0.01）。FGF5 - X1和FGF5 - X2显著增加β - catenin mRNA表达（P < 0.01）。所有三种FGF5可变剪接体均抑制DPCs增殖。综上所述，FGF5可变剪接体可调控与HF生长发育相关基因的表达谱，抑制DPCs增殖，并对家兔HF生长的调控产生影响。本研究为通过FGF5调控进一步探究家兔HF发育机制提供了思路。
