Drozdov-Tikhomirov L N, Skurida G I
Mol Biol (Mosk). 1977 Jul-Aug;11(4):843-53.
A quantitative kinetic model is suggested for the auto-regulated tryptophan synthesis by E. coli trp-operon system and for tryptophan excretion from the cell mediated by transport systems. Applications of the model for the calculation of several parameters characterizing tryptophan metabolism are considered. In order to explain experimental data it is suggested that a system of tryptophane excretion from the cell exists which is induced by high tryptophan concentrations. The rate of tryptophan excretion was studied as a function of various genetic effects (derepression or reduction of retroinhibition) as well as of changes in intracellular concentrations of substrates of tryptophan synthesis (chorismic acid and serine). Possible ways of making the cell to excrete markedly higher quantities of tryptophan without changing the genotype are discussed.
提出了一种定量动力学模型，用于大肠杆菌色氨酸操纵子系统的自动调节色氨酸合成以及由转运系统介导的色氨酸从细胞中的排泄。考虑了该模型在计算表征色氨酸代谢的几个参数方面的应用。为了解释实验数据，有人提出存在一种由高色氨酸浓度诱导的色氨酸从细胞中排泄的系统。研究了色氨酸排泄速率作为各种遗传效应（去阻遏或反馈抑制降低）以及色氨酸合成底物（分支酸和丝氨酸）细胞内浓度变化的函数。讨论了在不改变基因型的情况下使细胞显著排泄更多色氨酸的可能方法。
