Xu J, Li F, Tang X, Yu J, Liu X
Research Institute of Biochemical Engineering East China University of Science and Technology, Shanghai, China.
Chin J Biotechnol. 1997;13(1):17-23.
Based on the known molecular interactions of aporepressor, corepressor, and inducer, a mathematical model of product formation about the Trp operon regulated recombinant microbes has been proposed. The linear relation of the overall transcription rate constant and overall translation rate constant on the specific growth rate has been suggested. We used experimental data where engineered microorganisms express interferon (IFN). Decay constant of IFN, mRNA transcription rate constant, and IFN expression rate constant have been estimated. An equation related to IFN concentration in fermentation on specific growth rate and Trp concentration has been obtained. By application of the equation, the expression of IFN is calculated based on Trp concentration and specific growth rate in fermentation. Also, the optimum overall culture time can be determined.
基于已知的阻遏蛋白、辅阻遏物和诱导物的分子相互作用，提出了一种关于色氨酸操纵子调控重组微生物产物形成的数学模型。提出了总转录速率常数和总翻译速率常数与比生长速率的线性关系。我们使用了工程微生物表达干扰素（IFN）的实验数据。估算了IFN的衰变常数、mRNA转录速率常数和IFN表达速率常数。得到了一个关于发酵中IFN浓度与比生长速率和色氨酸浓度的方程。通过应用该方程，基于发酵中的色氨酸浓度和比生长速率计算IFN的表达。此外，还可以确定最佳的总培养时间。
