Acta Biochim Biophys Sin (Shanghai). 2023 Nov 25;55(11):1740-1748. doi: 10.3724/abbs.2023143.
Diverse splicing types in nuclear and chloroplast genes of protist have been recognized for decades. However, the splicing machinery responsible for processing nuclear precursor messenger RNA introns, including -splicing of the 5' terminal outron and spliced leader (SL) RNA, remains elusive. Here, we identify 166 spliceosomal protein genes and two snRNA genes from . by performing bioinformatics analysis from a combination of next-generation and full-length transcriptomic RNA sequencing (RNAseq) data as well as draft genomic data. With the spliceosomal proteins we identified in hand, the insensitivity of . to some splicing modulators is revealed at the sequence level. The prevalence of SL RNA-mediated -splicing is estimated to be more than 70% from our full-length RNAseq data. Finally, the splicing proteomes between . and its three evolutionary cousins within the same Excavata group are compared. In conclusion, our study characterizes the spliceosomal components in . and provides the molecular basis for further exploration of underlying splicing mechanisms.
几十年来，人们已经认识到原生生物核和叶绿体基因中的不同剪接类型。然而，负责加工核前体信使 RNA 内含子的剪接机制，包括 5' 端外显子和剪接前导 RNA (SL RNA) 的剪接，仍然难以捉摸。在这里，我们通过结合下一代和全长转录组 RNA 测序 (RNAseq) 数据以及基因组草案数据进行生物信息学分析，从. 中鉴定了 166 个剪接体蛋白基因和两个 snRNA 基因。有了我们在手中鉴定的剪接体蛋白，在序列水平上揭示了. 对一些剪接调节剂的不敏感性。根据我们的全长 RNAseq 数据，SL RNA 介导的剪接的普遍性估计超过 70%。最后，比较了. 与其在同一挖掘群内的三个进化同源物之间的剪接蛋白组。总之，我们的研究描述了. 中的剪接体成分，并为进一步探索潜在的剪接机制提供了分子基础。
