Secretariat of Agriculture, Department of Agricultural Diagnosis and Research - Postgraduate Program in Animal Health of the Institute for Veterinary Research Desidério Finamor, Livestock and Rural Development of Rio Grande do Sul, Eldorado do Sul, (RS), Brazil.
Department of Microbiology, State University of Londrina, Londrina, Brazil.
Vet Res Commun. 2024 Feb;48(1):381-390. doi: 10.1007/s11259-023-10217-8. Epub 2023 Sep 14.
Desirable characteristics of Staphylococcus sp., Streptococcus sp., Bacillus sp., Klebsiella sp., Escherichia coli, and Pseudomonas pseudoalcaligenes isolated from the trachea of healthy turkeys were evaluated as probiotic candidates in the search for new alternatives to solve antimicrobial resistance issues in poultry. In current study phenotypic and genotypic capacity to produce bacteriocin-like substances, efficacy to inhibit the growth of avian pathogens, susceptibility to antimicrobials of bacteria isolated from the respiratory microbiota of healthy turkeys, and the presence of virulence-associated genes (VAGs) predictors of Avian Pathogenic Escherichia coli (APEC) were evaluated. Nine E. coli and one Klebsiella sp. strains produced bacteriocin-like substances, and all harbored the cvaA gene. Some strains also showed antagonistic activity against APEC. Multidrug-resistant profile was found in 54% of the strains. Six strains of bacteriocin-like substances producing E. coli also harbored 3-5 VAGs. The study showed that two bacterial genuses (Klebsiella sp. and E. coli) present desirable probiotic characteristics. Our results identified strains with potential for poultry's respiratory probiotic.
从健康火鸡的气管中分离出的葡萄球菌属、链球菌属、芽孢杆菌属、克雷伯菌属、大肠杆菌和假单胞菌属的理想特性被评估为益生菌候选物，以寻找新的替代方案来解决家禽中的抗微生物药物耐药性问题。在本研究中，评估了从健康火鸡呼吸道微生物群中分离出的细菌的表型和基因型产生细菌素样物质的能力、抑制禽病原体生长的功效、对抗菌药物的敏感性以及与毒力相关的基因 (VAG)，这些基因是禽致病性大肠杆菌 (APEC) 的预测因子。9 株大肠杆菌和 1 株克雷伯菌产生细菌素样物质，所有菌株均携带 cvaA 基因。一些菌株还表现出对 APEC 的拮抗活性。发现 54%的菌株具有多药耐药性。产生细菌素样物质的 6 株大肠杆菌还携带 3-5 个 VAG。研究表明，两种细菌属（克雷伯菌属和大肠杆菌）具有理想的益生菌特性。我们的结果鉴定出了具有禽呼吸道益生菌潜力的菌株。
