Skurnik David, Clermont Olivier, Guillard Thomas, Launay Adrien, Danilchanka Olga, Pons Stéphanie, Diancourt Laure, Lebreton François, Kadlec Kristina, Roux Damien, Jiang Deming, Dion Sara, Aschard Hugues, Denamur Maurice, Cywes-Bentley Colette, Schwarz Stefan, Tenaillon Olivier, Andremont Antoine, Picard Bertrand, Mekalanos John, Brisse Sylvain, Denamur Erick
Division of Infectious Diseases, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA INSERM, IAME, UMR 1137, Paris, France Univ Paris Diderot, IAME, Sorbonne Paris Cité, UMR 1137, Faculté De Médecine, Site Xavier Bichat, Paris, France French National Reference Center for Bacterial Resistance in Commensal Flora, Laboratory of Bacteriology, Bichat-Claude Bernard Hospital, Assistance Publique-Hôpitaux De Paris, Paris, France
INSERM, IAME, UMR 1137, Paris, France Univ Paris Diderot, IAME, Sorbonne Paris Cité, UMR 1137, Faculté De Médecine, Site Xavier Bichat, Paris, France.
Mol Biol Evol. 2016 Apr;33(4):898-914. doi: 10.1093/molbev/msv280. Epub 2015 Nov 26.
In the context of the great concern about the impact of human activities on the environment, we studied 403 commensal Escherichia coli/Escherichia clade strains isolated from several animal and human populations that have variable contacts to one another. Multilocus sequence typing (MLST) showed a decrease of diversity 1) in strains isolated from animals that had an increasing contact with humans and 2) in all strains that had increased antimicrobial resistance. A specific B1 phylogroup clonal complex (CC87, Institut Pasteur schema nomenclature) of animal origin was identified and characterized as being responsible for the increased antimicrobial resistance prevalence observed in strains from the environments with a high human-mediated antimicrobial pressure. CC87 strains have a high capacity of acquiring and disseminating resistance genes with specific metabolic and genetic determinants as demonstrated by high-throughput sequencing and phenotyping. They are good mouse gut colonizers but are not virulent. Our data confirm the predominant role of human activities in the emergence of antimicrobial resistance in the environmental bacterial strains and unveil a particular E. coli clonal complex of animal origin capable of spreading antimicrobial resistance to other members of microbial communities.
在人们高度关注人类活动对环境影响的背景下，我们研究了从几个相互之间接触程度各异的动物和人群中分离出的403株共生大肠杆菌/大肠杆菌进化枝菌株。多位点序列分型（MLST）显示，多样性降低的情况出现在：1）从与人类接触日益增加的动物中分离出的菌株，以及2）所有抗微生物药物耐药性增加的菌株。鉴定并表征了一种源自动物的特定B1系统发育群克隆复合体（CC87，巴斯德研究所分类命名法），它是导致在人类介导的抗微生物药物压力较高的环境中分离出的菌株中观察到的抗微生物药物耐药性患病率增加的原因。如高通量测序和表型分析所示，CC87菌株具有获取和传播带有特定代谢和遗传决定因素的耐药基因的高能力。它们是良好的小鼠肠道定植菌，但不具毒性。我们的数据证实了人类活动在环境细菌菌株抗微生物药物耐药性出现过程中的主要作用，并揭示了一种源自动物的特定大肠杆菌克隆复合体，它能够将抗微生物药物耐药性传播给微生物群落的其他成员。
