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通过抑制 信号，促进结直肠癌（CRC）细胞的迁移和侵袭。

improves migration and invasion of colorectal cancer (CRC) cells through inhibiting signaling.

机构信息

Department of General Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning, China.

Department of Urology, Shengjing Hospital of China Medical University, Shenyang 110004, Liaoning, China.

出版信息

Aging (Albany NY). 2023 Sep 13;15(17):9182-9192. doi: 10.18632/aging.205027.

DOI:10.18632/aging.205027
PMID:37708299
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10522371/
Abstract

BACKGROUND

Kinesin family member 18A () is involved in the development of a variety of human malignancies. However, we have never known the influences of on colorectal cancer (CRC). The study is designed to investigate the effect and molecular mechanism of KIF18A on the progression of colorectal cancer.

METHODS

We have not only analyzed the database using GEO, but have examined the effect of on the development of CRC by subcutaneous tumorigenesis in nude mice. HE staining was used to observe tumor size. Besides, we make use of Western blotting to monitor the expression of related proteins. In addition, the scratch wound assay and Transwell assay were conducted to detect the effect of on the migration and invasion of CRC cells.

RESULTS

The results of GEO database analysis suggested that KIF18A had a positive correlation with the growth of CRC. The results of subcutaneous tumorigenesis and HE staining in nude mice explained that promoted the progression of CRC. Both scratch wound assay and Transwell indicated that the migration and invasion of CRC could be promoted by . The results of Western blot illustrated that could forward the migration and invasion of CRC cells, and inhibit , which promoted the activation of signaling pathway, thus bringing about the expression of and .

CONCLUSION

In conclusion, can further the activation of signaling pathway by means of inhibiting transcription. Therefore, it is inferred that that is a therapeutic target for CRC.

摘要

背景

驱动蛋白家族成员 18A () 参与多种人类恶性肿瘤的发生。然而，我们从未了解过对结直肠癌（CRC）的影响。本研究旨在探讨 KIF18A 对结直肠癌进展的影响及其分子机制。

方法

我们不仅使用 GEO 数据库进行了分析，还通过裸鼠皮下肿瘤生成实验研究了对 CRC 发生发展的影响。通过 HE 染色观察肿瘤大小。此外，我们利用 Western blot 监测相关蛋白的表达。另外，划痕实验和 Transwell 实验检测了对 CRC 细胞迁移和侵袭的影响。

结果

GEO 数据库分析结果表明，KIF18A 与 CRC 的生长呈正相关。裸鼠皮下肿瘤生成和 HE 染色结果表明促进了 CRC 的进展。划痕实验和 Transwell 实验表明，能够促进 CRC 的迁移和侵袭。Western blot 结果表明，能够促进 CRC 细胞的迁移和侵袭，并抑制的转录，从而激活 信号通路，导致的表达。

结论

综上所述，通过抑制的转录，进一步激活 信号通路。因此，可以推断是 CRC 的治疗靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/0bb1f80fa73f/aging-15-205027-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/4e41dcc74a82/aging-15-205027-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/803f9066efd8/aging-15-205027-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/ea533fe2f409/aging-15-205027-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/6b8430a493d3/aging-15-205027-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/1ef94b794032/aging-15-205027-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/e1a512031d48/aging-15-205027-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/0bb1f80fa73f/aging-15-205027-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/4e41dcc74a82/aging-15-205027-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/803f9066efd8/aging-15-205027-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/ea533fe2f409/aging-15-205027-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/6b8430a493d3/aging-15-205027-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/1ef94b794032/aging-15-205027-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/e1a512031d48/aging-15-205027-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ade8/10522371/0bb1f80fa73f/aging-15-205027-g007.jpg

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