improves migration and invasion of colorectal cancer (CRC) cells through inhibiting signaling.

BACKGROUND

Kinesin family member 18A () is involved in the development of a variety of human malignancies. However, we have never known the influences of on colorectal cancer (CRC). The study is designed to investigate the effect and molecular mechanism of KIF18A on the progression of colorectal cancer.

METHODS

We have not only analyzed the database using GEO, but have examined the effect of on the development of CRC by subcutaneous tumorigenesis in nude mice. HE staining was used to observe tumor size. Besides, we make use of Western blotting to monitor the expression of related proteins. In addition, the scratch wound assay and Transwell assay were conducted to detect the effect of on the migration and invasion of CRC cells.

RESULTS

The results of GEO database analysis suggested that KIF18A had a positive correlation with the growth of CRC. The results of subcutaneous tumorigenesis and HE staining in nude mice explained that promoted the progression of CRC. Both scratch wound assay and Transwell indicated that the migration and invasion of CRC could be promoted by . The results of Western blot illustrated that could forward the migration and invasion of CRC cells, and inhibit , which promoted the activation of signaling pathway, thus bringing about the expression of and .

CONCLUSION

In conclusion, can further the activation of signaling pathway by means of inhibiting transcription. Therefore, it is inferred that that is a therapeutic target for CRC.