全基因组鉴定硝酸盐转运蛋白 1/肽转运蛋白家族（NPF）基因表明，PaNPF5.5 在高硝酸盐条件下增强甜樱桃对硝酸盐的吸收。

Genome-wide identification of nitrate transporter 1/peptide transporter family (NPF) genes reveals that PaNPF5.5 enhances nitrate uptake in sweet cherry under high nitrate condition.

机构信息

School of Agriculture, Ludong University, Yantai 264025, China; College of Life Sciences, Ludong University, Yantai 264025, China.

College of Life Sciences, Ludong University, Yantai 264025, China.

出版信息

Gene. 2023 Dec 20;888:147797. doi: 10.1016/j.gene.2023.147797. Epub 2023 Sep 12.

DOI:10.1016/j.gene.2023.147797

PMID:37708922

Abstract

NITRATE TRANSPORTER 1 (NRT1)/PEPTIDETRANSPORTER (PTR) family (NPF) plays a significant role in nitrate transport. However, little is known about the NPF genes in sweet cherry. In this study, a total of 60 PaNPF genes in sweet cherry were identified by bioinformatics, which were divided into 8 families. Transcriptomic analysis showed that most PaNPF genes responded to both low and high nitrate conditions, especially PaNPF5.5, which was highly up-regulated under high nitrate condition. Molecular analysis showed that PaNPF5.5 was a transporter localized to the cell membrane. Further functional studies found that PaNPF5.5 overexpression promoted the growth of sweet cherry rootstock Gisela 6 by accelerating the nitrogen absorption process under high nitrate environment. Taken together, we believe that PaNPF5.5 plays an important role in regulating the transport of nitrate at high nitrate conditions, and provides a promising method for improving nitrate absorption efficiency at nitrogen excess environment.

摘要

硝酸盐转运蛋白 1（NRT1）/肽转运蛋白（PTR）家族（NPF）在硝酸盐转运中起着重要作用。然而，关于甜樱桃中的 NPF 基因知之甚少。本研究通过生物信息学方法鉴定了甜樱桃中的 60 个 PaNPF 基因，这些基因被分为 8 个家族。转录组分析表明，大多数 PaNPF 基因对低氮和高氮条件均有响应，特别是 PaNPF5.5，在高氮条件下高度上调。分子分析表明，PaNPF5.5 是一种定位于细胞膜的转运蛋白。进一步的功能研究发现，PaNPF5.5 的过表达通过在高硝酸盐环境下加速氮吸收过程，促进了甜樱桃砧木吉塞拉 6 的生长。综上所述，我们认为 PaNPF5.5 在高硝酸盐条件下调节硝酸盐的转运中起着重要作用，为在氮过剩环境下提高硝酸盐吸收效率提供了一种有前景的方法。