Department of Radiology, The Second Xiangya Hospital of Central South University, Changsha City, Hunan Province, 410011, China.
Department of Urology Surgery, Xiangya Hospital Central South University, Changsha City, Hunan Province, 410008, China.
Environ Res. 2023 Dec 1;238(Pt 1):117130. doi: 10.1016/j.envres.2023.117130. Epub 2023 Sep 13.
The use of multiparametric magnetic resonance imaging (MRI) to distinguish complex histopathological changes in liver fibrosis has not yet been systematically established. The purpose of this study is to gauge the efficacy of a cutting-edge MRI platform for evaluating ecotoxicologically hazardous carbon tetrachloride (CCl) induced liver fibrosis, while also scrutinizing the relationship between MRI and its histopathological features. Thirty-six mice were randomly divided into 6 groups, each with 6 mice. Control mice received an intraperitoneal injection of olive oil, while the experimental mice received different doses of intraperitoneal injection of CCl. Both sets underwent this process twice per week over a duration of 5 weeks. MRI measurements encompassed T1WI, T2WI, T1 mapping, T2 mapping, T2* mapping. Liver fibrosis and inflammation were assessed and classified using Metavir and activity scoring systems. CCl successfully induced liver fibrosis in mice, showing an increasing extent of liver fibrosis and liver function damage with the increasing dosage of CCl. Compared with the control group, T1, ΔT1, and T2 in the experimental group were considerably elevated (P < 0.05) than those in the control group. Spearman's correlation showed that the correlation of Native T1 and △T1 with fibrosis (r = 0.712, 0.678) was better than with inflammation (r = 0.688, 0.536). T2 correlation with inflammation (r = 0.803) was superior to fibrosis (r = 0.568). ROC analysis showed that the AUC of Native T1 was highest (0.906), followed by ΔT1 (0.852), while the AUC increased to 0.945 when all relevant MRI parameters were combined. T1 is the most potent MRI parameter for evaluating CCl-induced liver fibrosis, followed by ΔT1. Meanwhile, T2 may not be suitable for evaluating liver fibrosis but is more suitable for evaluating liver inflammation.
多参数磁共振成像（MRI）用于区分肝纤维化的复杂组织病理学变化尚未得到系统确立。本研究旨在评估一种先进的 MRI 平台评估生态毒性危险的四氯化碳（CCl）诱导的肝纤维化的疗效，同时检查 MRI 与其组织病理学特征之间的关系。36 只小鼠随机分为 6 组，每组 6 只。对照组小鼠接受腹腔注射橄榄油，实验组小鼠接受不同剂量的腹腔注射 CCl。两组均每周两次，共 5 周。MRI 测量包括 T1WI、T2WI、T1 映射、T2 映射、T2*映射。使用 Metavir 和活动评分系统评估和分类肝纤维化和炎症。CCl 成功诱导了小鼠肝纤维化，随着 CCl 剂量的增加，肝纤维化和肝功能损伤的程度逐渐增加。与对照组相比，实验组的 T1、ΔT1 和 T2 明显升高（P<0.05）。Spearman 相关性分析显示，Native T1 和 ΔT1 与纤维化的相关性（r=0.712、0.678）优于与炎症的相关性（r=0.688、0.536）。T2 与炎症的相关性（r=0.803）优于纤维化（r=0.568）。ROC 分析显示，Native T1 的 AUC 最高（0.906），其次是 ΔT1（0.852），当所有相关 MRI 参数结合时，AUC 增加至 0.945。T1 是评估 CCl 诱导的肝纤维化的最有效 MRI 参数，其次是 ΔT1。同时，T2 可能不适合评估肝纤维化，但更适合评估肝炎症。
