College of Ocean Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
School of Marine Engineering, Jimei University, Xiamen, 361021, China.
Anal Chim Acta. 2023 Oct 16;1278:341745. doi: 10.1016/j.aca.2023.341745. Epub 2023 Aug 21.
In this work, a "turn off-on" fluorescent sensor was developed for highly sensitive determination of tert-butylhydroquinone (TBHQ) based on an Fe(III)-based metal-organic framework (Fe-MOF). An Fe-MOF with an octahedral structure was synthesized via a simple hydrothermal method using ferric chloride hexahydrate and 2-aminoterephthalic acid (NH-BDC) as raw materials. The fluorescence of Fe-MOF is extremely weak owing to ligand-to-metal charge transfer (LMCT) and internal filtration effect (IFE). When the system contained TBHQ, the binding of TBHQ to Fe(III) inhibited the LMCT of the fluorescent ligand NH-BDC to Fe(III), releasing the fluorescence of NH-BDC and thus restoring the fluorescence. With this as the basis, a rapid, sensitive, and selective fluorescence sensor is developed for the detection of TBHQ. Under the optimal conditions, TBHQ showed good linearity with fluorescence intensity in the range of 0-1.5 × 10 μmol L and a detection limit of 0.0030 μmol L (S/N = 3). The selectivity, reproducibility, and stability of the developed Fe-MOF-based sensors are comprehensively studied. Finally, the practicality of the method is verified by examining the detection of TBHQ in soybean oil; the results are consistent with those obtained using conventional high-performance liquid chromatography.
在这项工作中，基于三价铁基金属有机骨架（Fe-MOF），开发了一种用于高灵敏度测定叔丁基对苯二酚（TBHQ）的“关-开”荧光传感器。采用简单的水热法，以六水合氯化铁和 2-氨基对苯二甲酸（NH-BDC）为原料，合成了具有八面体结构的 Fe-MOF。由于配体到金属电荷转移（LMCT）和内部过滤效应（IFE），Fe-MOF 的荧光非常微弱。当体系中含有 TBHQ 时，TBHQ 与 Fe(III) 的结合抑制了荧光配体 NH-BDC 向 Fe(III)的 LMCT，释放出 NH-BDC 的荧光，从而恢复了荧光。在此基础上，开发了一种快速、灵敏、选择性的荧光传感器用于 TBHQ 的检测。在最佳条件下，TBHQ 的荧光强度在 0-1.5×10 μmol L 的范围内呈良好线性，检测限为 0.0030 μmol L（S/N = 3）。对所开发的基于 Fe-MOF 的传感器的选择性、重现性和稳定性进行了综合研究。最后，通过检测大豆油中的 TBHQ 验证了该方法的实用性，结果与常规高效液相色谱法一致。
