International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu 214122, P. R. China.
Analyst. 2022 May 3;147(9):1906-1914. doi: 10.1039/d2an00265e.
-Butylhydroquinone (TBHQ) residues in foods pose a threat to human health. Therefore, it is necessary to develop a rapid method for TBHQ detection. In this study, a sensitive monoclonal antibody 5C3 (IgG2a subclass) against TBHQ was produced. It possessed a half maximal inhibitory concentration of 7.43 ng mL. A gold nanoparticle-based immunochromatographic assay (ICA) was established for the rapid and sensitive screening of TBHQ in soybean oil. Qualitative analysis results were obtained within 10 min and observed with the naked eye. The visual limit of detection (LOD) was 50 ng g and the cut-off value was 1000 ng g. A hand-held strip reader was used for quantitative analysis, in which the calculated LOD was defined as 18.68 ng g. The average recoveries of TBHQ ranged from 89.55% ± 2.70% to 100.66% ± 3.02% for soybean oil, with a coefficient of variation of 2.89%-7.05%. Therefore, our developed ICA is a useful tool for the rapid and on-site detection of TBHQ in real food samples.
丁基羟基茴香醚（TBHQ）在食品中的残留对人体健康构成威胁。因此，有必要开发一种快速检测 TBHQ 的方法。本研究制备了一种针对 TBHQ 的高灵敏单克隆抗体 5C3（IgG2a 亚类）。其半数最大抑制浓度（IC50）为 7.43ng/mL。建立了基于金纳米粒子的免疫层析分析（ICA）方法，用于快速灵敏地检测大豆油中的 TBHQ。定性分析结果可在 10min 内获得，并用肉眼观察。该方法的可视化检测限（LOD）为 50ng/g，截断值为 1000ng/g。使用手持式条带读取器进行定量分析，计算得出的 LOD 定义为 18.68ng/g。TBHQ 在大豆油中的平均回收率为 89.55%±2.70%至 100.66%±3.02%，变异系数为 2.89%-7.05%。因此，我们开发的 ICA 是一种用于快速现场检测实际食品样品中 TBHQ 的有用工具。
